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Fluorospot IL-4 positive control

  • GL
    Guest Leonie2020-11-16T10:48:41Z

    Hello,

    I would like to perform the IFNg/IL-4 Fluorospot on PBMCs stimulated with a vaccine antigen in order to see if the vaccine induces predominantly a Th1 or Th2 response. Do you have a recommenation for a positive control for IL-4 secretion? E.g. PBMCs of a patient with a specific helminth infection, malaria,...?

    Thanks!

    Leonie

  • C
    Christian@mabtech.com2020-11-16T11:51:21Z
    Admin

    Hello Leonie,

    For human IFNg/IL4 I would recommend using either (AntiCD3+antiCD28) or PHA. For IL-4 secretion it is good to go up 100K-150K cells/well in order to get good response.

    Combining antiCD3 and antiCD28 will give the response a push. Works also without but spots do become stronger and often more plentiful.

    For PHA I would recommend using the same cell numer.

    best,
    Christian