Just running a dual fluorospot IgG/IgM and can confirm spots using ELISpot but when I run Fluorospot for the same, I only have responses in the total IgG/IgM wells



	Any help please

I am Using the AID ELISpot reading machine

Hi Eunice



	I will gladly help you with you.



	However, I think that I need some more information about you assay first.



	As I understand it you are seeing antigen-specific IgG and IgM spots in ELISpot, but not in FluoroSpot when you run a dual IgG/IgM assay? Is that right?



	Since you see IgG and IgM spots in the total wells, there seems to be no problem with the detection antibodies or the cells.



	Please provide some more info on the protocols (you dont have to share specifics, only the general protocol that you use).



	Best regards



	Peter

Agree with Peter. But please also add some well images from the AID reader. Add them into a powerpoint and write in what is what. Then upload here. Will help a lot.

Forums

Spots in positive control and not antigen specific