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Can I use the same ELISA plate washer for FluoroSpot?

  • Qf
    Questions from customers2017-10-22T12:10:10Z

    Hi Mabtech,

    I am currently running Flurospot plates from Mabtech. I have a general question when it comes to washing plates for the Flurospot: Would you recommend to always use separate plate-washers for ELISA plates and Flurospot plates or would you say that it is overall ok to wash both ELISA plates and Flurospot plates with the same plate washer? 

    Thanks a lot in advance for your help 

    Best

    Sabrina

  • J
    Jens@mabtech.com2017-10-22T12:27:36Z
    Admin

    Hi Sabrina,

     

    The problem with using the same washer for both ELISA and FluoroSpot is that 1) the two methods normally use different buffers for washing (buffer with Tween for ELISA and without Tween for FluoroSpot) and 2) that you use different settings for how deep the tips go into the wells (should not go as deep in the membrane plates as in the regular ELISA plates). The latter can probably be handled via the software but then creates some room for mistakes. 

     

    So while possible to use the same washer for the two methods, it is much more convenient to use separate ones and, I think. It iswell worth the investment at least if you run a decent number of both ELISA and FluoroSpot. Depending on the type of washer you buy they are also not that expensive. We ourselves use one called ELx50 from Biotek Instruments that is not terribly sophisticated but very reliable.

     

    Kind regards,

    Jens

  • GA
    Guest Alora2022-04-07T22:47:48Z

    Hi Mabtech,

    I'm also planning to buy a plate washer for ELISA and FluoroSpot - since the previous reply was in 2017, do you have any new recommendations or are you still using the ELx50?  Are there any that you've heard don't work well for ELISpot?  Or are there specific features I should look for in the plate washer?

    Thanks,

    Alora

  • C
    Christian@mabtech.com2022-05-02T11:02:54Z
    Admin
    On 4/8/2022 at 12:47 AM, Guest Alora said:

    Hi Mabtech,

    I'm also planning to buy a plate washer for ELISA and FluoroSpot - since the previous reply was in 2017, do you have any new recommendations or are you still using the ELx50?  Are there any that you've heard don't work well for ELISpot?  Or are there specific features I should look for in the plate washer?

    Thanks,

    Alora

    Hi Alora, sorry we missed you. We sitll use Biotek washers. The newest ones we purchased are successors to the ELx50 and are now called 50 TS:

    https://www.biotek.com/products/liquid-handling-microplate-washers/50-ts-washer/

    Works very well.