So totally new to ELISpot so I have no idea what I'm doing. I'm trying to design an experiment to detect in IFN-γ from T cells. My subjects are dogs. Where do I start?
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CD8 T cells & IFN gamma
- GMGuest Mia2016-09-29T00:37:13Z
- CChristian@mabtech.com2016-09-29T05:36:25ZAdmin
Dear Mia, you have come to the right place! ELISpot is an excellent choice for detecting IFN-g secretion by CD8 T-cells in dogs.
ELISpot is not a difficult method, especially if you use our kits called ELISpotPLUS. These come pre-coated with capture antibody so you are essentially guaranteed an optimal assay. All you need to think about is isolating your dog PBMCs in a good way, what cell culture medium to use, which antigens to test and at what number of cells per well to incubate.
It is always good to start with some pilot experiments were you can titrate you cells and antigen to see where the optimum response can be found.
We have had a Dog IFN-g kit for some time now. It can be found here:
http://www.mabtech.com/products/3113-4apw-2_canine-ifn-%CE%B3-elispotplus-alp
Nonetheless, we are actually to day launching a new, in-house developed system to Dog IFN-g. We can send you a sample plate for you to evaluate the system and technique. The launch letter is attached.
best,
Christian
- CChristian@mabtech.com2016-09-29T09:07:27ZAdmin
Hey again Mia,
This morning in Sweden we updated our Canine product line so they are now based on our in-house monoclonal antibodies. Just so you now. The link above is still valid but it goes to our new updated kit, not the previous one.
-Christian