Catalog / IL-12/-23 (p40) antibodies
IL-12/-23 (p40) antibodies
We make 7 IL-12/-23 (p40) antibodies for 2 species, unconjugated or conjugated to biotin. This page explains how they differ, so you can pick the one your experiment needs.
Rather use a ready-made kit? See our IL-12/-23 p40 ELISA kits and IL-12/-23 p40 ELISpot kits.
What differs between these antibodies
Which species they are for
Available for Human and Mouse. The species decides which clones there are, so it is the first thing to choose. Many also cross-react with related species, so if you work in one we do not list here, check the reactivity on the product page.
Which clone
We make 6 IL-12/-23 (p40) antibody clones. A clone is one specific antibody that binds one site on its target, so it is what you cite or reorder by name. A sandwich assay needs two clones that bind different sites; the capture and detection pairs below are matched for that.
Which conjugate
Unconjugated antibodies carry no label. You coat a plate with them as the capture antibody, or use them where the antibody itself is the reagent, such as for neutralization. Biotinylated antibodies are detected with a streptavidin conjugate, such as streptavidin-ALP or streptavidin-HRP.
Which formulation
Some come azide-free. Sodium azide preserves the standard formulation but is toxic to cells, so choose the azide-free version for work with live cells.
Capture and detection pairs
To build your own IL-12/-23 (p40) ELISA or ELISpot, coat with the unconjugated capture antibody and detect with the biotinylated detection antibody, then a streptavidin conjugate. These are the same pairs our own kits use.
| Species | Capture | Detection | For |
|---|---|---|---|
| HumanELISA, ELISpot | MT86/221 | MT618, biotin | ELISA, ELISpot |
| MouseELISA, ELISpot | C15.6 | C17.8, biotin | ELISA, ELISpot |
About IL-12/-23 (p40)
Interleukin 12 (IL-12) is, when biologically active, a heterodimer (p70) consisting of two covalently linked subunits, p35 and p40. IL-12 is produced by antigen-presenting cells, specifically dendritic cells, and macrophages. IL-12 is a proinflammatory cytokine that promotes Th1-type responses by inducing IFN-γ production and enhancing the proliferation and cytotoxicity of NK and T cells.
Interleukin-23 (IL-23) is a disulfide-linked heterodimer that consists of a unique p19 subunit and the p40 subunit of IL-12. IL-23 is a proinflammatory cytokine, which is produced by macrophages and dendritic cells upon infections with certain pathogens. The IL-23 receptor is expressed on Th17 cells, and IL-23 is responsible for Th17 expansion and stabilization.
Since both IL-12 and IL-23 heterodimers comprise the p40 subunit, capture immunoassays based on antibodies to p40 will detect both cytokines. Such assays can also detect p40 monomers and dimers.
Find the right IL-12/-23 (p40) antibody
Choose what your experiment needs. When your choices match a single antibody, we'll link you to its product page for prices and ordering.
Species
What you are measuring in
Application
What you will use it for
Conjugate
Unlabelled or labelled
Formulation
Preservative and concentration
Clone
If you already know which
Start by choosing a species.
Related reading
Guides and tutorials covering IL-12/-23 (p40) and how to use these antibodies.
