Catalog / Mouse IL-4 ELISA kits

Mouse IL-4 ELISA kits

Pick the right ELISA kit for Mouse IL-4. Our kits differ in what we put in the box and how you develop the signal – this page explains what those differences mean.

IL-4 ELISA kits are also available for human.

What differs between these kits

Whether a pre-coated plate is included

Some of our kits arrive with the plate already coated with the capture antibody, so you can start with your samples. Others supply the antibodies and standard only. Coating your own plate is more work, and it gives you control over the coating conditions and the plate.

Which detection system

Available with Streptavidin-ALP or Streptavidin-HRP. You add the streptavidin conjugate as a second incubation after the biotinylated antibody, which amplifies the signal. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) perform equivalently, so that choice usually comes down to the substrate and reader you already work with.

Supplied without a plate, streptavidin conjugate

With a pre-coated strip plate, streptavidin conjugate, 1 plate

With a pre-coated strip plate, streptavidin conjugate, 2 plates

With a pre-coated strip plate, streptavidin conjugate, 10 plates

About IL-4

These Mouse IL-4 ELISA kits target Il4 (Mus musculus, Gene ID 16189).

Interleukin 4 (IL-4) is produced primarily by Th2 cells, mast cells, eosinophils, and basophils. IL-4 was first identified to costimulate B cell growth; the cytokine is essential for B cell secretion of IgE and augments the production of IgG1 in vivo. In addition to regulating B cell growth and immunoglobulin secretion, IL-4 also affects T cells. In vitro, IL-4 promotes T cell growth and can induce cytolytic T cell activity. IL-4 also induces the differentiation of naive CD4 T cells into Th2 cells, which are characterized by their capacity to secrete the cytokines IL-4, IL-5, and IL-10 upon activation, while simultaneously inhibiting the generation of Th1 cells.