Catalog / Human CD25 ELISA kits
Human CD25 ELISA kits
Pick the right ELISA kit for Human CD25. Our kits differ in how you develop the signal – this page explains what those differences mean.
What differs between these kits
Which detection system
Available with Streptavidin-ALP or Streptavidin-HRP. You add the streptavidin conjugate as a second incubation after the biotinylated antibody, which amplifies the signal. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) perform equivalently, so that choice usually comes down to the substrate and reader you already work with.
Supplied without a plate, streptavidin conjugate
About CD25 (IL-2Rα)
These Human CD25 ELISA kits target IL2RA (Homo sapiens, Gene ID 3559).
CD25 is expressed by activated T cells, B cells, and monocytes. Tregs, responsible for regulating immune tolerance, express the highest amounts of CD25. CD25 is the IL-2 receptor α chain, which functions as one subunit of the high-affinity variant of the IL-2 receptor. When cleaved off by proteases, the soluble receptor (sIL-2Rα) is released extracellularly. Soluble CD25 has a low affinity for IL-2 and can function either as a decoy receptor reducing the activity of IL-2 or by enabling trans presentation to cells expressing the dimeric, intermediate-affinity IL-2R. Increased levels of soluble CD25 indicate ongoing immune responses, and therefore CD25 has been investigated as a biomarker for disease. For example, elevated sCD25 levels have been associated with autoimmune disorders, severe COVID-19, and some types of cancer.
| Also known as | CD25, cluster of differentiation 25, Interleukin-2 receptor subunit alpha, IL2RA, Tac |
| Produced by | T cell |
| Reactivity | Human |
| Gene ID | 3559 |
Find the right kit
Choose what your experiment needs. When your choices match a single kit, we'll link you to its product page for prices and ordering.
Kit detection system
Which enzyme conjugate we supply
Choose a detection system to continue.
Frequently bought together
What customers usually order alongside these kits.
Related reading
Guides and tutorials covering Human CD25 and the ELISA method.
