Catalog / CD25 (IL-2Rα) antibodies
CD25 (IL-2Rα) antibodies
We make 4 CD25 (IL-2Rα) antibodies for human, unconjugated or conjugated to biotin. This page explains how they differ, so you can pick the one your experiment needs.
Rather use a ready-made kit? See our CD25 ELISA kits.
What differs between these antibodies
Which clone
We make 4 CD25 (IL-2Rα) antibody clones. A clone is one specific antibody that binds one site on its target, so it is what you cite or reorder by name. A sandwich assay needs two clones that bind different sites; the capture and detection pairs below are matched for that.
Which conjugate
Unconjugated antibodies carry no label. You coat a plate with them as the capture antibody, or use them where the antibody itself is the reagent. Biotinylated antibodies are detected with a streptavidin conjugate, such as streptavidin-ALP or streptavidin-HRP.
Capture and detection pairs
To build your own CD25 (IL-2Rα) ELISA or ELISpot, coat with the unconjugated capture antibody and detect with the biotinylated detection antibody, then a streptavidin conjugate. These are the same pairs our own kits use.
| Species | Capture | Detection | For |
|---|---|---|---|
| HumanELISA | MTAW11A | MTAW9, biotin | ELISA |
About CD25 (IL-2Rα)
CD25 is expressed by activated T cells, B cells, and monocytes. Tregs, responsible for regulating immune tolerance, express the highest amounts of CD25. CD25 is the IL-2 receptor α chain, which functions as one subunit of the high-affinity variant of the IL-2 receptor. When cleaved off by proteases, the soluble receptor (sIL-2Rα) is released extracellularly. Soluble CD25 has a low affinity for IL-2 and can function either as a decoy receptor reducing the activity of IL-2 or by enabling trans presentation to cells expressing the dimeric, intermediate-affinity IL-2R. Increased levels of soluble CD25 indicate ongoing immune responses, and therefore CD25 has been investigated as a biomarker for disease. For example, elevated sCD25 levels have been associated with autoimmune disorders, severe COVID-19, and some types of cancer.
| Also known as | CD25, cluster of differentiation 25, Interleukin-2 receptor subunit alpha, IL2RA, Tac |
| Produced by | T cell |
| Species | Human |
| Gene ID | Homo sapiens: 3559 |
Find the right CD25 (IL-2Rα) antibody
Choose what your experiment needs. When your choices match a single antibody, we'll link you to its product page for prices and ordering.
Application
What you will use it for
Conjugate
Unlabelled or labelled
Clone
If you already know which
Next, choose what you will use it for.
Related reading
Guides and tutorials covering CD25 (IL-2Rα) and how to use these antibodies.
