Catalog / Anti-IgM antibodies
Anti-IgM antibodies
We make 5 antibodies against IgM for 2 species, unconjugated or conjugated to biotin or ALP. This page explains how they differ, so you can pick the one your experiment needs.
Rather use a ready-made kit? See our IgM ELISA kits and IgM ELISpot kits.
What differs between these antibodies
Which species they are for
Available for Human and Mouse. This is the species whose immunoglobulin you want to detect, and it decides which clones there are, so it is the first thing to choose.
Which clone
We make 4 anti-IgM antibody clones. A clone is one specific antibody that binds one site on its target, so it is what you cite or reorder by name. A sandwich assay needs two clones that bind different sites; the capture and detection pairs below are matched for that.
Which conjugate
Unconjugated antibodies carry no label. You coat a plate with them as the capture antibody, or use them where the antibody itself is the reagent. Biotinylated antibodies are detected with a streptavidin conjugate, such as streptavidin-ALP or streptavidin-HRP. ALP-conjugated antibodies carry the enzyme themselves, so no streptavidin step is needed.
Capture and detection pairs
To build your own IgM ELISA or ELISpot, coat with the unconjugated capture antibody and detect with the biotinylated detection antibody, then a streptavidin conjugate. These are the same pairs our own kits use.
| Species | Capture | Detection | For |
|---|---|---|---|
| HumanELISA, ELISpot | MT11/12 | MT22, biotin | ELISA, ELISpot |
| MouseELISA, ELISpot | MT6A3 | MT9A2, biotin | ELISA, ELISpot |
About IgM
Immunoglobulin M (IgM) is the first antibody isotype (also called class) secreted by the adaptive immune system in response to a foreign antigen. Plasma B cells secrete IgM mainly as pentamers, i.e., five IgM molecules joined together by the J chain. Because IgM is formed early in the immune response and is later replaced by IgG, specific antibodies of the IgM class are characteristic of a recent, ongoing, or chronic infection. Therefore, IgM represents the isotype with the lowest affinity for antigens. Due to its structure, with 10 antigen-binding sites per IgM pentamer, it can nevertheless bind surface-bound antigens with very high avidity. Immunoglobulin M constitutes approximately 10% of the total immunoglobulin concentration in serum. Resting mature yet naive, B lymphocytes express monomeric IgM as a transmembrane antigen receptor that functions as part of the B cell receptor (BCR).
Find the right anti-IgM antibody
Choose what your experiment needs. When your choices match a single antibody, we'll link you to its product page for prices and ordering.
Species
Whose antibodies you detect
Application
What you will use it for
Conjugate
Unlabelled or labelled
Clone
If you already know which
Start by choosing a species.