Catalog / Anti-IgG antibodies
Anti-IgG antibodies
We make 20 antibodies against IgG for 7 species, unconjugated or conjugated to biotin, HRP, or ALP. This page explains how they differ, so you can pick the one your experiment needs.
Rather use a ready-made kit? See our IgG ELISA kits and IgG ELISpot kits.
What differs between these antibodies
Which species they are for
Available for Human, Mouse, Bovine, Equine, Hamster, Porcine, and Rabbit. This is the species whose immunoglobulin you want to detect, and it decides which clones there are, so it is the first thing to choose.
Which clone
We make 16 anti-IgG antibody clones. A clone is one specific antibody that binds one site on its target, so it is what you cite or reorder by name. A sandwich assay needs two clones that bind different sites; the capture and detection pairs below are matched for that.
Which conjugate
Unconjugated antibodies carry no label. You coat a plate with them as the capture antibody, or use them where the antibody itself is the reagent. Biotinylated antibodies are detected with a streptavidin conjugate, such as streptavidin-ALP or streptavidin-HRP. HRP and ALP-conjugated antibodies carry the enzyme themselves, so no streptavidin step is needed.
Capture and detection pairs
To build your own IgG ELISA or ELISpot, coat with the unconjugated capture antibody and detect with the biotinylated detection antibody, then a streptavidin conjugate. These are the same pairs our own kits use.
| Species | Capture | Detection | For |
|---|---|---|---|
| HumanELISpot | MT91/145 | MT78/145, biotin | ELISpot |
| BovineELISA, ELISpot | MT134 | MT391, biotin | ELISA, ELISpot |
| EquineELISA | MT329A4 | MT310A61, biotin | ELISA |
| HamsterELISA | MTAE142 | MTAE913, biotin | ELISA |
| PorcineELISpot | MT421 | MT424, biotin | ELISpot |
| RabbitELISA, ELISpot | MT5131 | MT536, biotin | ELISA, ELISpot |
About IgG
Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.
Find the right anti-IgG antibody
Choose what your experiment needs. When your choices match a single antibody, we'll link you to its product page for prices and ordering.
Species
Whose antibodies you detect
Application
What you will use it for
Conjugate
Unlabelled or labelled
Clone
If you already know which
Start by choosing a species.
Related reading
Guides and tutorials covering IgG and how to use these antibodies.
