FociSpot Path: Influenza A (NP)

FociSpot Path: Influenza A (NP)

4405-4AT-1

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Content

Detection mAb19C10, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus
Plate96-well tissue culture treated plate

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This kit is intended for FociSpot immunostaining of the influenza A nucleoprotein, which enables enumeration of influenza A virus foci in focus forming assays (FFA). For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

FociSpot
In FociSpot, virus foci are detected by using mAbs specific to viral proteins. After fixation and permeabilization of infected cells in a 96-well plate, the virus-specific biotinylated detection mAb is added. The use of Streptavidin-ALP enzyme conjugate, followed by a precipitating substrate enables detection of foci. Foci can be counted manually, but counting with Mabtech IRIS 2 is rapid and offers easy data handling.

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Product details

ProductFociSpot Path: Influenza A (NP)
ApplicationFociSpot
AnalyteInfluenza A (NP)
Specificity

Influenza A nucleoprotein (NP)

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibody, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.

FociSpot immunostaining of influenza A nucleoprotein in infected MDCK cells. MDCK cells were infected with influenza A virus (titrated, virus dilutions 2,000x, 4,000x, and 8,000x are shown). After fixation and permeabilization, foci were detected using anti-influenza A (NP) mAb 19C10, followed by Streptavidin-ALP and BCIP/NBT-plus substrate. Foci were counted using the Apex software in Mabtech IRIS 2.

Datasheet/Protocol
SDS

Featured product, Published October 5, 2023

Influenza A FociSpot kit and monoclonal antibody
Measure the amount of infectious particles and determine the levels of neutralizing antibodies.

Tutorial, Published October 12, 2023

Step-by-step guide to FociSpot
FociSpot is our new assay platform that includes both kits and a brand-new analysis capability of Mabtech IRIS™ 2 allowing for automated counting of foci in a 96-well plate format. Let us show you how you can run an FRNT50 with FociSpot.
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Influenza A (NP)

Analyte description

The Nucleoprotein (NP) is a structural protein and encapsidates the negative strand RNA of influenza A into ribonucleoproteins (RNP). NP plays a role in replication, transcription, RNA packaging, and nuclear trafficking. The NP does not only interact with the RNA, but also the viral polymerase, the M protein, and several host factors. 

Alternative namesNucleoprotein, NP (of influenza A)