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Anti-human/monkey TNF-α mAb (MT15B15), PF647P

Detection of Rhesus macaque TNF-α by flow cytometry using PF647P conjugated mAb MT15B15 (Mabtech).

Anti-human/monkey TNF-α mAb (MT15B15), PF647P

3512M-72-100T

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Isotype control mAb (MT128) for mouse IgG1, PF647P

Intended use

This monoclonal antibody enables specific detection of monkey TNF-α-producing cells in flow cytometry.

Serum/Plasma samples

Recommendation

5 µl is recommended for staining of 1 million cells in a total volume of 50 µl. PF647P is excited by the red laser (633 nm). The excitation max is 654 nm and the emission max is 672 nm.

Product details

ProductAnti-human/monkey TNF-α mAb (MT15B15), PF647P
ApplicationFlow cytometry
AnalyteTNF-α
AntibodyMT15B15
ConjugatePF647P
ClonalityMonoclonal
ImmunogenRecombinant human TNF-α
HostMouse
IsotypeIgG1
ReactivityNon-human primates
Specificity

Native TNF-α from rhesus and cynomolgus macaques. Native and recombinant human TNF-α.

Cross-reactivity

For information on reactivity with other non-human primates (NHP), please check the NHP cross-reactivity guide.

Supplied in

PBS with 0.2% BSA and 0.09% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MT15B15, conjugated to PF647P fluorophore. Ready to use.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8 °C.

Shelf lifeAt least 12 months from date of receipt.

Detection of Rhesus macaque TNF-α by flow cytometry using PF647P conjugated mAb MT15B15 (Mabtech).
Detection of TNF-α by flow cytometry in viable lymphocytes from Rhesus macaque. Stimulated, fixed and permeabilized cells were stained with anti-human/monkey mAb MT15B15-PF647P (left panel). Matched isotype control antibody (right panel). Flow cytometry was performed on a BD FACSVerse system.

Datasheet/ProtocolSDS

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TNF-α

Analyte description

Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Alternative namesTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF-alpha, TNFA, TNFSF2, TNLG1F
Cell typeT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID7124

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Anti-human/monkey TNF-α mAb (MT15B15), PF488P