Anti-human IL-4 mAb (IL4-II), biotin

Anti-human IL-4 mAb (IL4-II), biotin

3410-6-250

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Content

Monoclonal antibody IL4-II, biotinylated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

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Anti-human IL-4 mAb (IL4-I), unconjugated
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Product specifications

Intended use

This monoclonal antibody enables specific detection of human IL-4 in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples

Recommendation

IL4-II is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb IL4-I (product code 3410-3).

Product details

ProductAnti-human IL-4 mAb (IL4-II), biotin
ApplicationELISpot, FluoroSpot, ELISA
AnalyteIL-4
AntibodyIL4-II
ConjugateBiotin
ClonalityMonoclonal
ImmunogenRecombinant human IL-4
HostMouse
IsotypeIgG1
ReactivityHuman, Non-human primates
Specificity

Native and recombinant human IL-4

Cross-reactivityCross-reacts with IL-4 from rhesus and cynomolgus macaques. For more information please check the Cross-reactivity guide section below.
Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration1 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody IL4-II, biotinylated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

IL-4

Analyte description

Interleukin 4 (IL-4) is produced primarily by Th2 cells, mast cells, eosinophils, and basophils. IL-4 was first identified to costimulate B cell growth; the cytokine is essential for B cell secretion of IgE and augments the production of IgG1 in vivo. In addition to regulating B cell growth and immunoglobulin secretion, IL-4 also affects T cells. In vitro, IL-4 promotes T cell growth and can induce cytolytic T cell activity. IL-4 also induces the differentiation of naive CD4 T cells into Th2 cells, which are characterized by their capacity to secrete the cytokines IL-4, IL-5, and IL-10 upon activation, while simultaneously inhibiting the generation of Th1 cells.

Alternative namesInterleukin 4, IL-4, IL4, BCGF-1, BSF-1, BCGF1, BSF1
Cell typeT cell, Th2, Treg
Gene ID3565
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