Anti-human IFN-α mAb (detection), biotin

Standard curve for human IFN-α in bead-based multiplex.

Anti-human IFN-α mAb (detection), biotin

5317-6-100

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Anti-human IFN-α mAb (capture), unconjugated

Intended use

This monoclonal antibody enables specific detection of human IFN-α in bead-based multiplex assays. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Anti-human IFN-α mAb (detection) is recommended in combination with Anti-human IFN-α mAb (capture) (product code 5317-3) in bead-based multiplex.

Product details

Product	Anti-human IFN-α mAb (detection), biotin
Application	Bead-based multiplex
Analyte	IFN-α pan
Antibody	Anti-human IFN-α mAb (detection)
Conjugate	Biotin
Clonality	Monoclonal
Reactivity	Human
Specificity	Native and recombinant human IFN-α. In combination with Anti-human IFN-α mAb (detction), subtypes 1/13, 2, 4, 5, 6, 7, 8, 10, 14, 16 and 17 are detected.
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Anti-human IFN-α mAb (detection), biotin. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C.
Shelf life	At least 12 months from date of receipt.

Representative standard curve for human IFN-α in bead-based multiplex.

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IFN-α pan

Analyte description

Interferon-α and interferon-β are type I interferons. These antiviral cytokines are mainly produced by virus-infected cells after sensing the presence of danger signals such as viral DNA or RNA. IFN-α and IFN-β act through the same receptor and initiate an anti-viral response, affect immune regulation, T cell responses, and also anti-tumor effects have been shown. A key producer of type I interferons is the plasmacytoid dendritic cell. Additionally, IFN-α is secreted by lymphocytes, macrophages, fibroblasts, and dendritic cells.

In humans, interferon-α has 13 subtypes: IFN-α 1, 2, 4, 5, 6, 7, 8, 10, 13, 14, 16, 17, and 21.

In mice, there are 14 IFN-α subtypes: 1, 2, 4, 5, 6T, 7/10, 8/6, 9, 11, 12, 13, 14, 15 (A), and B. There are also different allelic forms of mouse IFN-α subtypes 1, 7/10, 8/6, and 11 in mouse strains 129/Sv and C57BL/6.

Alternative names

Interferon-α, Interferon-alpha, IFN-α, IFN-alpha, IFN-a, IFNA