Anti-human apoH mAb (H219), unconjugated

Anti-human apoH mAb (H219), unconjugated

3711-3-250

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Content

Monoclonal antibody H219, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Apo ELISA buffer
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  • Code: 3652-M2
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Product specifications

Intended use

This monoclonal antibody enables specific detection of apoH in immunoassays such as ELISA and Western blot.

Serum/Plasma samples

Recommendation

H219 is recommended as capture mAb in ELISA in combination with detection mAb H464 (product code 3711-6). The antibody is also suitable for Western blot.

Product details

ProductAnti-human apoH mAb (H219), unconjugated
ApplicationELISA, Western blot
AnalyteapoH
AntibodyH219
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenNative human apoH
HostMouse
IsotypeIgG1
ReactivityHuman, Non-human primates
Specificity

Human apolipoprotein H (apoH)

Cross-reactivityCross-reacts with apoH from rhesus and cynomolgus macaques. For more information please check the Cross-reactivity guide section below.
Purification

Purified from in vitro cultures by protein G affinity chromatography.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody H219, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
Detection of human apoH with mAb H219 in Western blot. Human plasma (0.1 µl and 0.01 µl) in SDS sample buffer were denatured at 70˚C for 10 minutes and run on SDS-page / Western blot. ApoH was detected using 1 µg/ml H219 (biotinylated) followed by SA-ALP and BCIP/NBT-plus substrate.
Datasheet/ProtocolSDS

apoH

Analyte descriptionApolipoprotein H (apoH, also known as apolipoproteinH, apo H, beta 2-glycoprotein 1 and β2-glycoprotein I) is associated to VLDL, HDL and chylomicrons in plasma and binds to anionic lipids e.g. phospholipids. ApoH is the dominant target of autoantibodies in anti-phosphlipid syndrome (APS). In the blood coagulation pathway, apoH is involved both as an anti-coagulant and as a procoagulant. Normal concentration of apoH in human plasma is approximately 0.05-0.6 g/L.
Alternative names, B2G1, B2GP1, BG
Gene ID350
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Tutorial, Published November 14, 2022

Apolipoprotein dilution
Recommendations for Mabtech's apolipoprotein ELISAs