Anti-equine IFN-γ mAb (MT166), PF488P
Anti-equine IFN-γ mAb (MT166), PF488P
Intended use
This monoclonal antibody enables specific detection of equine IFN-γ-producing cells in flow cytometry.
Serum/Plasma samples
Recommendation
5 µl is recommended for staining of 1 million cells in a total volume of 50 µl. PF488P is excited by the blue laser (488 nm). The excitation max is 490 nm and the emission max is 516 nm.
Product details
| Product | Anti-equine IFN-γ mAb (MT166), PF488P |
| Application | Flow cytometry |
| Analyte | IFN-γ |
| Antibody | MT166 |
| Conjugate | PF488P |
| Clonality | Monoclonal |
| Immunogen | Recombinant equine IFN-γ |
| Host | Mouse |
| Isotype | IgG2a |
| Reactivity | Horse |
| Specificity | Native and recombinant equine (horse) IFN-γ |
| Supplied in | PBS with 0.2% BSA and 0.09% sodium azide. Sterile-filtered (0.2 µm). |
| Contents | Monoclonal antibody MT166, conjugated to PF488P fluorophore. Ready to use. |
Shipping and Storage
| Shipping | Shipped at ambient temperature. |
| Storage | Store product at 4-8 °C. |
| Shelf life | At least 12 months from date of receipt. |
Detection of IFN-γ by flow cytometry in viable equine PBMC. Cells were stimulated for 16 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were then fixed and permeabilized using 4% paraformaldehyde and saponin, and subsequently stained with MT166-PF488P (solid line). Matched isotype control antibody (dashed line). The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.
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