Anti-bovine TNF-α mAb (MT771), biotin

Anti-bovine TNF-α mAb (MT771), biotin

3012-6-250

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Content

Monoclonal antibody MT771, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Delivery 4-9 business days

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Anti-bovine TNF-α mAb (MTF4918), unconjugated


Product specifications

Intended use

This monoclonal antibody enables specific detection of bovine TNF-α in immunoassays such as ELISpot, FluoroSpot, ELISA, and flow cytometry.

Serum/Plasma samples

Recommendation

MT771 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb MTF4918 (product code 3012-3).

Product details

ProductAnti-bovine TNF-α mAb (MT771), biotin
ApplicationELISpot, FluoroSpot, ELISA
AnalyteTNF-α
AntibodyMT771
ConjugateBiotin
ClonalityMonoclonal
Immunogen

Recombinant bovine TNF-α

HostMouse
IsotypeIgG1
ReactivityCow
Specificity

Native and recombinant bovine (cow) TNF-α

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MT771, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Find out which analyte combinations we have evaluated in T cell FluoroSpot and which combinations are affected by capture effects or not. 

What is a capture effect?

When capture antibodies with different specificities are coated together, capture of one cytokine may affect the secretion of other cytokines. This is usually more pronounced when studying T cell responses with polyclonal stimuli compared to antigen-specific responses. Capture effects are seldom a problem and can often be counteracted by addition of an anti-CD28 antibody.

Featured product, Published September 14, 2023

Detect TNF-α secretion from cow cells
You can now analyze the secretion of bovine TNF-α with ELISA, ELISpot, and FluoroSpot.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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TNF-α

Analyte description

Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Alternative namesTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, TNF-a-alpha
Cell typeT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID280943