Anti-bovine IL-4 mAb (bIL4-II), biotin

Anti-bovine IL-4 mAb (bIL4-II), biotin

3118-6-250

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Validated for our assays.

Content

Monoclonal antibody bIL4-II, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Delivery 4-9 business days

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Anti-bovine IL-4 mAb (bIL4-I), unconjugated


Product specifications

Intended use

This monoclonal antibody enables specific detection of bovine and sheep IL-4 in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples

Recommendation

bIL4-II is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb bIL4-I (product code 3118-3).

Product details

ProductAnti-bovine IL-4 mAb (bIL4-II), biotin
ApplicationELISpot, FluoroSpot, ELISA
AnalyteIL-4
AntibodybIL4-II
ConjugateBiotin
ClonalityMonoclonal
ImmunogenRecombinant bovine IL-4
HostMouse
IsotypeIgG2b
ReactivityCow, Sheep
Specificity

Native and recombinant bovine (cow) IL-4

Cross-reactivity

Cross-reacts with IL-4 from sheep. For more information, please check the Veterinary cross-reactivity guide.

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody bIL4-II, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Find out which analyte combinations we have evaluated in T cell FluoroSpot and which combinations are affected by capture effects or not. 

What is a capture effect?

When capture antibodies with different specificities are coated together, capture of one cytokine may affect the secretion of other cytokines. This is usually more pronounced when studying T cell responses with polyclonal stimuli compared to antigen-specific responses. Capture effects are seldom a problem and can often be counteracted by addition of an anti-CD28 antibody.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IL-4

Analyte description

Interleukin 4 (IL-4) is produced primarily by Th2 cells, mast cells, eosinophils, and basophils. IL-4 was first identified to costimulate B cell growth; the cytokine is essential for B cell secretion of IgE and augments the production of IgG1 in vivo. In addition to regulating B cell growth and immunoglobulin secretion, IL-4 also affects T cells. In vitro, IL-4 promotes T cell growth and can induce cytolytic T cell activity. IL-4 also induces the differentiation of naive CD4 T cells into Th2 cells, which are characterized by their capacity to secrete the cytokines IL-4, IL-5, and IL-10 upon activation, while simultaneously inhibiting the generation of Th1 cells.

Alternative namesInterleukin 4, IL-4, IL4, BCGF-1, BSF-1
Cell typeT cell, Th2, Treg
Gene ID280824, 443327
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ELISpot Plus: Bovine IL-4 (ALP)