ELISA Pro: Human Thioredoxin-1

ELISA Pro: Human Thioredoxin-1

3580-1HP-1

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Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.

Components

Detection mAbMT13X3, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant human thioredoxin-1 ELISA standard
SubstrateELISA substrate: TMB for HRP
PlatePre-coated ELISA strip plate (mAb MT17R6)
Also providedAdhesive plate covers
Buffers/SolutionsAssay buffer
Standard reconstitution buffer
Streptavidin-HRP diluent
Wash buffer concentrate
Stop solution

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Product specifications

Intended use

This ELISA Pro kit is intended for the quantification of reduced and oxidized forms of human Thioredoxin-1 (Trx1) in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

The ELISA diluent included in this kit blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in other species. The ELISA diluent has been validated using from healthy human blood donors.

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Product details

ProductELISA Pro: Human Thioredoxin-1
ApplicationELISA
AnalyteThioredoxin-1
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human Thioredoxin-1 (Trx1). Reduced and oxidized forms of Trx1 are detected.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
Standard range31.2-2000 pg/ml
Sensitivity15 pg/ml

The limit of detection is the lowest concentration that is possible to detect but not necessarily quantify with precision and accuracy.

Representative standard curve for human Thioredoxin-1 (Trx1) ELISA Pro kit using TMB substrate.
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Thioredoxin-1

Analyte description

Thioredoxin-1 (Trx1) is an oxidoreductase protein and thus may act as an anti-oxidant by facilitating the reduction of cysteine residues of other proteins. Besides keeping the intracellular milieu in a reduced state and being involved in the defence against oxidative stress, thioredoxin-1 specifically regulates several transcription factors and receptors. Increased plasma levels of thioredoxin-1 has been associated with several psychiatric disorders.

Alternative namesThioredoxin-1, Trx1, TRDX, TRX, TRX1, Trx80
Cell typeT cell, Tc, Th1, Th2, Th17, Treg, Monocyte/MΦ
Gene ID7295