FluoroSpot Plus: Human IFN-γ/IL-2

FSP-0102-2

Publications: 46

Documents

Questions?

Ensure reproducibility when diving into a multiplex analysis.

Components

Detection mAbs	Anti-IFN-γ mAb (7-B6-1), BAM
	Anti-IL-2 mAb (MT8G10), biotin
Fluorophore conjugates	Anti-BAM mAb, 490
	SA-550
Positive control	Anti-CD3 mAb (CD3-2)
Co-stimulus	Anti-CD28 mAb (CD28-A)
Buffer/Solution	FluoroSpot enhancer
Plate	Pre-coated FluoroSpot plate (mAbs 1-D1K and MT2A91/2C95)

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

1 / 2

PepPool: CEF (CD8), human

Product specifications

Intended use

This FluoroSpot Plus kit is intended for the enumeration of cells secreting human IFN-γ and IL-2 using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	FluoroSpot Plus: Human IFN-γ/IL-2
Application	FluoroSpot
Analyte	IFN-γ, IL-2
Reactivity	Human
Specificity	This kit is based on matched pairs of mAbs specific for human IFN-γ and IL-2.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Performance

PBMC (250,000 cells/well) were incubated overnight in the absence or presence of the CEF peptide pool (available from Mabtech, code 3616-1) and the number of cells secreting IFN-γ and/or IL-2 was analyzed by FluoroSpot. Double-secreting cells were determined as spots having the same center point in an image overlay of LED490 (IFN-γ) and LED550 (IL-2) images. Spot analysis was made with Mabtech IRIS.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

Product highlights

Highlighted research, Published May 17, 2022

Third vaccine dose important for T cell response against Omicron

Three doses mRNA vaccine had better T cell responses than recipients of two doses. Mabtech IRIS and FluoroSpot Flex: Human IFN-γ/TNF-α/IL-2/Granzyme B were used to assess T cell immunity in mRNA vaccine recipients.

Highlighted research, Published December 16, 2021

SARS-CoV-2-specific immune responses in patients with autoimmune diseases

Patients with rheumatic diseases showed T cell responses comparable to healthy control. Patients with MS and after mRNA vaccination show different memory B cell IgG responses. SARS-CoV-2 specific immune responses in autoimmunity was analyzed using FluoroSpot, ELISpot Path, and Mabtech IRIS.

Highlighted research, Published December 2, 2021

Importance of studying SARS-CoV-2-specific memory B cells

When studying antibodies in circulation, remember that memory B cells might hide out of sight. In this study, detection of SARS-CoV-2-specific T and memory B cells 6 and 15 months after infection.

Highlighted research, Published November 24, 2021

Polyfunctional T cells with Mabtech IRIS

Study of development and persistence of virus-specific cellular and humoral immune responses, during the acute phase and two follow-ups after COVID-19 disease in hospitalized patients. Mabtech’s FluoroSpot Plus: Human IFN-γ/IL-2/TNF-α were analyzed with Mabtech IRIS.

Highlighted research, Published April 5, 2019

FluoroSpot more sensitive than flow cytometry

FluoroSpot assay detected cytokine-secreting cells even when as few as 10 transfected cells were added to the cell suspension. In contrast, at least 5 000 transfected cells were required to detect the cytokines by flow cytometry.

Highlighted research, Published May 29, 2018

FluoroSpot helps elucidate the pleiotropic effects of lenalidomide

Mabtech FluoroSpot Plus: IFN-γ/IL-2/TNF-α was utilized to show the polyfunctional profile of CD8+ T cells in patients with multiple myeloma after lenolidomide treatment.

Publications (46)

Loading publications...

Analyte information

IFN-γ

Analyte description	Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Alternative names	Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell type	T cell, Tc, Th1, NK cell
Gene ID	3458

IL-2

Analyte description	Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases.
Alternative names	Interleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, TCGF, lymphokine
Cell type	T cell, Th1, Th2, Th17, Tfh
Gene ID	3558