FluoroSpot Path: TB (ESAT-6+CFP-10+EspC) Human IFN-γ/IL-2

FluoroSpot Path: TB (ESAT‑6+CFP-10+EspC) Human IFN-γ/IL-2


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Paves your way to functional analysis of a specific research question.


PlatePre-coated FluoroSpot plate (mAbs 1-D1K and MT2A91/2C95)
Detection mAbsAnti-IFN-γ mAb (7-B6-1), BAM
Anti-IL-2 mAb (MT8G10), biotin
Fluorophore conjugatesAnti-BAM mAb, 490
Buffer/SolutionFluoroSpot enhancer
Positive controlAnti-CD3 mAb (CD3-2)
Co-stimulusAnti-CD28 mAb (CD28-A)
Peptide poolsTB (ESAT-6), scanning
TB (CFP-10), scanning
TB (EspC), scanning

In stock

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Product specifications

Intended use

This FluoroSpot Path kit is intended for the enumeration of cells secreting human IFN-γ and IL-2 in response to TB peptides using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Peptide pool composition
The scanning pools consist of synthetic peptides of 15-mers overlapping with 11 amino acids, covering the complete proteins. The ESAT-6 scanning pool is derived from the early secretory antigenic target protein and contains 21 peptides. The CFP-10 scanning pool is derived from the culture filtrate protein and contains 23 peptides. Finally, the EspC scanning pool is derived from the ESX-1 secretion-associated protein and contains 23 peptides.


Product details

ProductFluoroSpot Path: TB (ESAT-6+CFP-10+EspC) Human IFN-γ/IL-2
AnalyteIFN-γ, IL-2

This kit is based on matched pairs of mAbs specific for human IFN-γ and IL-2.

Shipping and Storage


Shipped at ambient temperature.


Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Peptide pools should be stored at -20 °C or below. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
Human PBMC (250,000 cells/well) from an individual with latent tuberculosis were incubated overnight in the absence or presence of the ESAT-6 and CFP-10 scanning pools (Mabtech). The number of cells secreting IFN-γ and/or IL-2 was analyzed by FluoroSpot. Single cytokine secreting cells as well as double-secreting cells were determined. Spot center was used to identify spots from double-secreting cells, visualized in an image overlay of LED490, FITC (IFN-γ) and LED550,Cy3 (IL-2). Spot analysis was made with Mabtech IRIS.

Featured product, Published September 2, 2021

Our TB peptide pools go Path
Our PepPools for tuberculosis were combined into ELISpot and FluoroSpot Path kits.
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Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458


Analyte description

Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases. 

Alternative namesInterleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, TCGF, lymphokine
Cell typeT cell, Th1, Th2, Th17, Tfh
Gene ID3558