ELISpot Plus: Human TNF-α (HRP)

3512-4HPW-2

Publications: 17

Documents

Questions?

The easiest way to achieve reproducibility in ELISpot.

Components

Detection mAb	MT20D9, biotin
Enzyme conjugate	Streptavidin-HRP
Substrate	ELISpot substrate: TMB for HRP
Plate	Pre-coated ELISpot plate, white MSIP (mAb MT25C5)

In stock

Delivery 4-9 business days

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Complementary products

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PepPool: CEF (CD8), human

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human TNF-α using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Plus: Human TNF-α (HRP)
Application	ELISpot
Analyte	TNF-α
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human TNF-α.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf life	At least 18 months from date of receipt.

Performance

Human PBMC were incubated overnight with LPS (8,000 cells/well) or medium only (25,000 cells/well) and the number of cells secreting TNF-α was analyzed by ELISpot.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published September 7, 2023

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

Publications (17)

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Analyte information

TNF-α

Analyte description	Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.
Alternative names	Tumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF-alpha, TNFA, TNFSF2, TNLG1F
Cell type	T cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID	7124