iPSC-derived hepatocytes for drug identification and to study cellular pathophysiology
Cayo et al. have made an impressive work using new techniques such as iPSCs and humanized mice, to show that cardiac glycosides reduces hepatocyte production of apoB. They use apoB ELISA from Mabtech to analyze apoB secreted from iPSC-derived hepatocytes, and apoB in serum of avatar mice. They have also used the anti-apoB LDL17/20 for immunoprecipitation and Western Blot. Their article was published in April 2017 in the renowned journal Cell Stem Cell, and in the same edition, two other publications, Pashos et al. and Warren et al., are also analyzing secreted apoB from iPSC-derived hepatocytes using Mabtech’s apoB ELISA.
Liu et al. and Yang et al. are two additional recent papers studying inherited disorders with iPSC-derived hepatocytes, and like in the other articles, the authors are also using Mabtech’s apoB ELISA.Friday, June 16, 2017
Use of R848 and IL-2 to stimulate memory B cells
In 2013, Jahnmatz, M et al published a newly developed protocol for polyclonal activation of human memory B cells. The authors showed the superior functionality of stimulating B cells using the TLR7/8-agonist R848 and IL-2 compared to the other stimulation protocols used at the time. Since then, we include R848 and IL-2 in all of our human immunoglobulin ELISpot and FluoroSpot kits.
The article is now one of the most highly cited papers in Journal of Immunological Methods.Thursday, June 15, 2017
Characterization of Wilms’ tumor specific T cells with FluoroSpot
Danielzik et al. utilized Mabtech’s IFN-γ/Granzyme B FluroSpot kit to detect among others rare, specific, and double secreting T cells against Wilms’ tumor. The study honors high sensitivity, small sample size and the possibility to detect three different cell times in one FluoroSpot assay!Tuesday, June 13, 2017
IL-8 secretion in cattle after BoHV-4 infection
Premiere for our bovine IL-8 ELISA kit in the Reference-section. The study of Chanrot et al. demonstrates the susceptibility of bovine endometrial epithelial cells to bovine herpes virus 4 (BoHV-4), leading to increased IL-8 secretion. BoHV-4 is transmitted through breeding and is associated with abortion and chronic infertility in cows.Tuesday, May 30, 2017
CMV monitoring with IFN-gamma ELISpot
In addition, the assay proved to have low intra-assay, inter-assay, inter-operator and inter-site variability, and high linearity, sensitivity and specificity.Wednesday, May 24, 2017
Glycosylation differentiate latent TB from active
Look at this: Lu et al just published a paper in which they identify differential antibody glycosylation profiles that distinguish latent tuberculosis from active.Tuesday, September 27, 2016
FluoroSpot suitable for clinical trials
Looked at through Mabtech-colored glasses, this new paper by Körber et al highlights the superior robustness, simplicity and sensitivity of FluoroSpot vs Flow Cytometry. In addition, the data presented demonstrate that Mabtech’s IFNγ/IL-2 FluoroSpot kit has:
- Low replicate-to-replicate, plate-to-plate and day-to-day variability.
- High specificity.
- Good linearity.
- Good correlation with ELISpot assays.
Conclusion: The IFNγ/IL-2 FluoroSpot assay meets requirements of standardized assay procedure and data analysis, making it suitable in clinical trial settings.Friday, September 16, 2016
ELISpot in ferret model of influenza
Authors DiPiazza et al recently confirmed the specificity and utility of different monoclonal antibodies in the ferret-model for influenza.
The authours used ELISpot and flow cytometry to study T cell responses to infection and their results facilitate future studies of e.g. new vaccine candidates and the role of T cells in virus transmission.
Yes, some of those “different monoclonal antibodies” mentioned above are Mabtech-products.Tuesday, September 13, 2016