New applications and formats for Mabtech’s human IL-21 mAbs
We are happy to introduce new applications for our IL-21 monoclonal antibodies.
- Retain IL-21 in cell culture supernatants for improved detection in ELISA
- Neutralize IL-21 bioactivity
IL-21 is an important regulating cytokine affecting both innate and adaptive immune responses. To enable detection of IL-21, Mabtech provides both ELISA and ELISpot kits.
During cell culture, secreted IL-21 may be consumed by IL-21R-bearing cells, rendering it difficult to detect by ELISA. To overcome this and thus increase detectable IL-21 levels in cell culture supernatants, Mabtech’s biotinylated mAb MT21.3m can be included during cell culture1. This mAb will bind IL-21 and prevent it from interacting with its receptor, effectively keeping it in solution to be quantified by ELISA (Fig. 1).
In addition, we have launched a mixture of mAbs (MT216G/21.3m) with the ability to neutralize IL-21 bioactivity on human cells naturally expressing the IL-21 receptor1 (Fig. 2).
Please note that the mAbs MT21.3m and MT216G are referred to as MT3 and MT6, respectively, in the article below.
- anti-human IL-21 mAb MT21.3m, biotinylated, AF
- anti-human IL-21 mAbs MT216G/21.3m, for Neutralization