Frequently Asked Questions
Which ELISpot plates do you recommend?
We recommend 96-well ELISpot plates with PVDF membrane that efficiently bind high amounts of capture antibody provided the membrane is pre-treated (i.e. activated) with ethanol.
ELISpot plates in different variants are supplied by Mabtech as supplementary products. Please look in the web shop.
Read more about plate characteristics, pros and cons in the Plate coating guide.
Why should ELISpot plates be pre-treated with ethanol prior to coating?The ELISpot plate membrane has a porous hydrophobic structure. To allow high amounts of capture antibody to bind, the membrane should be treated with ethanol to make it hydrophilic. This permits the dense antibody coating that is necessary for obtaining high quality spots. The ethanol-treatment should be performed according to the instructions in the Plate coating guide.
How should I dilute the ELISA standard?The standard range of the assay found in the ELISA protocol should be used as a guideline for the standard curve. The idea is that your standard dilutions should cover the standard range which is indicated in the product insert. Dilutions should be made no more than 30 minutes prior to the experiment. Please find more details in the ELISA analysis/ELISA standard.
Why do serum and plasma samples require a special dilution buffer?Human serum/plasma samples often contain heterophilic antibodies which by binding to the capture and the detection antibodies can give a similar signal as the analyte. The use of ELISA diluent or Assay buffer will reduce this unspecific binding and is strongly recommended for ELISA analysis of serum and plasma samples. Please read more in the ELISA analysis/Reducing unspecific signal guidelines.