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3450-2A

Human IL-12/-23 (p40) ELISpot BASIC (ALP)

  • Human IL-12/-23 (p40) ELISpot (ALP)
  • Human IL-12/-23 (p40) ELISpot (ALP)

Basic ELISpot kit for enumeration of cells secreting IL-12/-23 (p40)

The human IL-12/-23 (p40) ELISpot kit is ideal for users who want the flexibility of setting up their own assay. The kit enables enumeration of cells secreting human IL-12/-23 (p40), and contains a matched pair of monoclonal capture and detection antibodies and Streptavidin-ALP.

The monoclonal antibodies in this kit are specific to the p40 subunit of IL-12 and IL-23. ELISpot assays based on antibodies to p40 will detect both IL-12 and IL-23. Such assays can also detect p40 monomers and dimers.

Human IL-12/-23 (p40) ELISpot kits are also available with Streptavidin-HRP. See Related products.

As complementary products, we offer ELISpot plates and substrates. See Related products.

Species cross-reactivity

The monoclonal antibodies in this kit cross-react with IL-12/-23 (p40) from non-human primates. See the Human/NHP cross-reactivity table.


Analyte Information

IL-12/-23 (p40)
Interleukin-12 (IL-12, IL12) is, when biologically active, a heterodimer (p70) consisting of two covalently linked subunits, p35 and p40. IL-12 is produced by antigen-presenting cells, specifically dendritic cells and macrophages. IL-12 is a proinflammatory cytokine that promotes Th1-type responses by inducing IFN-γ production and enhancing proliferation and cytotoxicity of NK and T cells. Interleukin-23 (IL-23) is a disulfide-linked heterodimer which consists of a unique p19 subunit and the p40 subunit of IL-12. IL-23 is a proinflammatory cytokine, which is produced by macrophages and dendritic cells upon infections with certain pathogens. The IL-23 receptor is expressed on Th17 cells and IL-23 is responsible for Th17 expansion and stabilization. Since both IL-12 and IL-23 heterodimers comprise the p40 subunit, capture immunoassays based on antibodies to p40 will detect both cytokines. Such assays can also detect p40 monomers and dimers.

Ordering Information

Orders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.

Email:
Send your order to order@mabtech.com.

Customers from USA or Canada:
Please send your order to mabtech.usa@mabtech.com.
Application:
  • ELISpot
Reactivity:
  • Human / NHP
Documentation: Product datasheet

Product SDS
Contents:
  • Capture mAbs (MT86/221)
  • Biotinylated detection mAb (MT618)
  • Streptavidin-ALP
Size: for 4 plates
Other formats: Click to view all Human/NHP IL-12/-23 (p40) products
  • Non-Human Primate Cross-reactivity

    The systems reactive with NHPs are either based on cross-reactive human kits or specifically developed monkey kits (NHP).

    Cross-reactivity verification tests have been performed in ELISpot and/or ELISA by Mabtech and/or by others. Assays with less evidence of cross-reactivity are defined as indications (symbols within parentheses).

     

      Rhesus Macaque Cynomolgus Macaque Pig-tailed Macaque Baboon* Sooty Mangabey African Green Monkey Night Monkey* Common Marmoset Squirrel Monkey
    ASSAY Old world New world
      Rhesus Macaque Cynomolgus Macaque Pig-tailed Macaque Baboon* Sooty Mangabey Afr. Green Monkey Aotus* Common Marmoset Squirrel Monkey
    Assay Old world New world
    ApoA1           
    ApoB              
    ApoE (NHP)              
    ApoE            
    ApoH          
    GM-CSF ( ) ( )      
    Granzyme A                
    Granzyme B          
    IFN-α2                
    IFN-α pan       ( )      
    IFN-γ (NHP)
    IFN-γ ( )
    IgA (NHP)              
    IgA              
    IgG              
    IgM              
    Insulin            
    IL-1β                
    IL-2 (NHP)
    IL-4 ( ) ( ) ( ) ( )
    IL-5 ( ) ( ) ( )  
    IL-6 ( ) ( )   ( )  
    IL-8 (NHP)                
    IL-8                
    IL-10              
    IL-12/-23 (p40)            
    IL-12 (p70)                
    IL-13 ( ) ( ) ( )    
    IL-17A (NHP) ( ) ( )      
    IL-17A          
    IL-21            
    IL-22                
    IL-23          
    IL-31                
    Perforin ( )      
    TGF-β1 (latent)              
      Rhesus Macaque Cynomolgus Macaque Pig-tailed Macaque Baboon* Sooty Mangabey Afr. Green Monkey Aotus* Common Marmoset Squirrel Monkey
                       
    SINGLE mAbs Old world New world
    SINGLE mAbs                  
    Cell stimulation Old world New world
    Cell stimulation                  
    CD3, mAb CD3-1              
    CD3, mAb CD3-2              
    CD28, mAb CD28-A              
    Flow cytometry Old world New world
    Flow cytometry                  
    Granzyme B, mAb GB11                
    IFN-γ, mAb 1-D1K ( ) ( ) ( ) ( ) ( ) ( )
    IL-2 mAb MT8G10 ( ) ( ) ( ) ( )   ( ) ( ) ( )
    IL-17A mAb MT504                
    Perforin mAb Pf-344          
    Ig reagents Old world New world
    Ig reagents                  
    IgG1 mAb MT1939              
    IgG2 mAb H6200              
    IgG3 mAb MTG34              
    IgG4 mAb MTG42              
    Western blot Old world New world
    Western blot                  
    IFN-γ, mAb MT111W ( ) ( ) ( ) ( ) ( ) ( ) ( ) ( ) ( )

    Symbol keys:

    Cross-reactivity

    Verified by Mabtech and/or by others. Tests performed in ELISpot and/or ELISA.

    • good
    • poor
    • no
    Indications of Cross-reactivity

    Based on reports from others or by analysis using recombinant proteins.

    • ( ) good
    • ( ) poor
    • ( ) no
    Blank boxes

    Represent cross-reactivities not evaluated.

    * Comprises several species. Cross-reactivity may have to be verified on a species basis.

    View human/NHP cross-reactivity table

  • Assay principle

    The ELISpot assay explained step-by-step
  • Image Gallery

    A library of representative ELISpot and FluoroSpot results.
  • Analyte Information

    • IL-12/-23 (p40)
      Interleukin-12 (IL-12, IL12) is, when biologically active, a heterodimer (p70) consisting of two covalently linked subunits, p35 and p40. IL-12 is produced by antigen-presenting cells, specifically dendritic cells and macrophages. IL-12 is a proinflammatory cytokine that promotes Th1-type responses by inducing IFN-γ production and enhancing proliferation and cytotoxicity of NK and T cells. Interleukin-23 (IL-23) is a disulfide-linked heterodimer which consists of a unique p19 subunit and the p40 subunit of IL-12. IL-23 is a proinflammatory cytokine, which is produced by macrophages and dendritic cells upon infections with certain pathogens. The IL-23 receptor is expressed on Th17 cells and IL-23 is responsible for Th17 expansion and stabilization. Since both IL-12 and IL-23 heterodimers comprise the p40 subunit, capture immunoassays based on antibodies to p40 will detect both cytokines. Such assays can also detect p40 monomers and dimers.
  • Ordering Information

    Orders to MabtechOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.


    Email:
    Send your order to order@mabtech.com.

    Customers from USA or Canada:
    Please send your order to mabtech.usa@mabtech.com.
  • Find out more

    Visit our Knowledge Center or see the FAQs.