|Anti-IFN-γ mAb (7-B6-1), BAM
|Anti-IL-5 mAb (5A10), biotin
|Anti-BAM mAb, 490
|Anti-CD3 mAb (CD3-2)
|Anti-CD28 mAb (CD28-A)
|Pre-coated FluoroSpot plate (mAbs 1-D1K and TRFK5)
Delivery 4-9 business days
This FluoroSpot Plus kit is intended for the enumeration of cells secreting human IFN-γ and IL-5 using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.
|FluoroSpot Plus: Human IFN-γ/IL-5
This kit is based on matched pairs of mAbs specific for human IFN-γ and IL-5.
Shipping and Storage
Shipped at ambient temperature.
Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.
|At least 12 months from date of receipt.
Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
|Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
|T cell, Tc, Th1, NK cell
Interleukin 5 (IL-5) is a pleiotropic cytokine primarily produced by T and NK cells. IL-5 specifically controls the survival, differentiation, and chemotaxis of eosinophils. But initially, IL-5 was characterized by its ability to support the growth and terminal differentiation of B cells.
|Interleukin-5, IL-5, IL5, BCGF-2, EDF, TRF
|T cell, Th2, NK cell