ELISpot Path: TB (ESAT‑6​+​CFP‑10​+​EspC) Human IFN‑γ

PBMC (250,000 cells/well) from an individual with latent tuberculosis were incubated overnight in the absence or presence of the ESAT-6, CFP-10, and EspC scanning pools (Mabtech) and the number of cells secreting IFN-γ was analyzed by ELISpot.
PBMC (250,000 cells/well) from a patient with tuberculosis infection were incubated overnight in the absence or presence of the ESAT-6 scanning pool and the number of cells secreting IFN-γ was analyzed by ELISpot.

ELISpot Path: TB (ESAT‑6​+​CFP‑10​+​EspC) Human IFN‑γ

3420-2AST-P4-1

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Bulk or custom format
A straightforward setup for functional analysis of a specific scientific question.

Components

PlatePre-coated ELISpot plate, strip (mAb 1-D1K)
Detection mAb7-B6-1, ALP
SubstrateBCIP/NBT-plus substrate for ELISpot
Positive controlAnti-CD3 mAb (CD3-2)
Peptide poolsTB (ESAT-6), scanning
TB (CFP-10), scanning
TB (EspC), scanning

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Path kit is intended for the enumeration of cells secreting human IFN-γ in response to TB peptides using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Peptide pool composition
The scanning pools consist of synthetic peptides of 15-mers overlapping with 11 amino acids, covering the complete proteins. The ESAT-6 scanning pool is derived from the early secretory antigenic target protein and contains 21 peptides. The CFP-10 scanning pool is derived from the culture filtrate protein and contains 23 peptides. Finally, the EspC scanning pool is derived from the ESX-1 secretion-associated protein and contains 23 peptides.

Recommendation

Product details

ProductELISpot Path: TB (ESAT-6+CFP-10+EspC) Human IFN-γ
ApplicationELISpot
AnalyteIFN-γ
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IFN-γ.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C. Peptide pools should be stored at -20 °C or below. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
PBMC (250,000 cells/well) from an individual with latent tuberculosis were incubated overnight in the absence or presence of the ESAT-6, CFP-10, and EspC scanning pools (Mabtech) and the number of cells secreting IFN-γ was analyzed by ELISpot.
PBMC (250,000 cells/well) from an individual with latent tuberculosis were incubated overnight in the absence or presence of the ESAT-6, CFP-10, and EspC scanning pools (Mabtech) and the number of cells secreting IFN-γ was analyzed by ELISpot.
PBMC (250,000 cells/well) from an individual with latent tuberculosis were incubated overnight in the absence or presence of the ESAT-6, CFP-10, and EspC scanning pools (Mabtech) and the number of cells secreting IFN-γ was analyzed by ELISpot.
PBMC (250,000 cells/well) from a patient with tuberculosis infection were incubated overnight in the absence or presence of the ESAT-6 scanning pool and the number of cells secreting IFN-γ was analyzed by ELISpot.
Datasheet/ProtocolSDS

Highlighted research
Published: October 28, 2024 | 18 minute read

Immunoassays for infectious disease research

Explore the role of immunoassays like ELISpot and FluoroSpot in infectious disease research. Discover how Mabtech's immunoassays enable researchers to study immune responses at the cellular level for diseases like TB, SARS-CoV-2, Malaria, HIV, and more.

Highlighted research
Published: October 28, 2024 | 3 minute read

ELISpot vs ELISA: Superior sensitivity in TB diagnostics

ELISpot is preferred over ELISA in TB diagnostics due to its higher sensitivity, enabling early detection of TB-specific T cells through single-cell analysis of IFN-γ secretion. ELISpot offers a more accurate immune response assessment, crucial for diagnosing TB in humans and animals.

Highlighted research
Published: December 21, 2023 | 1 minute read

White paper: Tuberculosis diagnosis with spots

Early and precise diagnosis is crucial for effective TB management, and ELISpot is increasingly being used for this purpose. This white paper examines the advantages of ELISpot in TB diagnosis and suggests future antigens and biomarkers that can enhance diagnostic accuracy.

Webinar
Published: November 17, 2023 | 1 minute read

Webinar: Novel insights into TB vaccines and diagnostics

Recording now available!

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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458
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FluoroSpot Path: TB (ESAT-6+CFP-10+EspC) Human IFN-γ/IL-2