ELISpot Path: TB (ESAT-6+CFP-10+EspC) Human IFN-γ

ELISpot Path: TB (ESAT-6+CFP-10+EspC) Human IFN-γ

3420-2AST-P4-1

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A straightforward setup for functional analysis of a specific scientific question.

Components

Detection mAb7-B6-1, ALP
SubstrateBCIP/NBT-plus
PlatePre-coated ELISpot plate, strip (mAb 1-D1K)
Positive controlAnti-CD3 mAb (CD3-2)
Peptide poolsTB (ESAT-6), scanning
TB (CFP-10), scanning
TB (EspC), scanning

In stock

Delivery 4-9 business days

Shipping $0.00

Product specifications

Intended use

This ELISpot Path kit is intended for the enumeration of cells secreting human IFN-γ in response to TB peptides using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Peptide pool composition
The scanning pools consist of synthetic peptides of 15-mers overlapping with 11 amino acids, covering the complete proteins. The ESAT-6 scanning pool is derived from the early secretory antigenic target protein and contains 21 peptides. The CFP-10 scanning pool is derived from the culture filtrate protein and contains 23 peptides. Finally, the EspC scanning pool is derived from the ESX-1 secretion-associated protein and contains 23 peptides.

Recommendation

Product details

ProductELISpot Path: TB (ESAT-6+CFP-10+EspC) Human IFN-γ
ApplicationELISpot
AnalyteIFN-γ
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IFN-γ.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C. Peptide pools should be stored at -20 °C or below. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
PBMC (250,000 cells/well) from an individual with latent tuberculosis were incubated overnight in the absence or presence of the ESAT-6, CFP-10, and EspC scanning pools (Mabtech) and the number of cells secreting IFN-γ was analyzed by ELISpot.
Datasheet/ProtocolSDS
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