|Detection mAb||7-B6-1, ALP|
|Plate||Pre-coated ELISpot plate, strip (mAb 1-D1K)|
|Positive control||Anti-CD3 mAb (CD3-2)|
|Peptide pools||TB (ESAT-6), scanning|
|TB (CFP-10), scanning|
|TB (EspC), scanning|
Delivery 4-9 business days
This ELISpot Path kit is intended for the enumeration of cells secreting human IFN-γ in response to TB peptides using the ELISpot assay. For research use only. Not for use in diagnostic procedures.
Peptide pool composition
The scanning pools consist of synthetic peptides of 15-mers overlapping with 11 amino acids, covering the complete proteins. The ESAT-6 scanning pool is derived from the early secretory antigenic target protein and contains 21 peptides. The CFP-10 scanning pool is derived from the culture filtrate protein and contains 23 peptides. Finally, the EspC scanning pool is derived from the ESX-1 secretion-associated protein and contains 23 peptides.
|Product||ELISpot Path: TB (ESAT-6+CFP-10+EspC) Human IFN-γ|
This kit is based on a matched pair of mAbs specific for human IFN-γ.
Shipping and Storage
Shipped at ambient temperature.
Store antibodies, enzyme conjugate, and substrate at 4-8 °C. Peptide pools should be stored at -20 °C or below. Plates may be kept at room temperature.
|Shelf life||At least 18 months from date of receipt.|