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ELISpot Flex: Human IL‑17A/F (ALP)

PBMC were incubated for 48 h in the absence (250,000 cells/well) or presence (50,000 cells/well) of anti-CD3 monoclonal antibody (available from Mabtech, code 3605-1-50) and the number of cells secreting IL-17A/F was analyzed by ELISpot.

ELISpot Flex: Human IL‑17A/F (ALP)

3523-2A

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Bulk or custom format
Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMTF411
Detection mAbMT504, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot

In stock

Delivery 4-9 business days

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Complementary products
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ELISpot substrate: BCIP/NBT-plus for ALP

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human IL-17A/F using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Human IL-17A/F (ALP)
ApplicationELISpot
AnalyteIL-17A/F
ReactivityHuman
SpecificityThis kit is based on a matched pair of mAbs enabling specific detection of human IL-17A/F.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.

PBMC were incubated for 48 h in the absence (250,000 cells/well) or presence (50,000 cells/well) of anti-CD3 monoclonal antibody (available from Mabtech, code 3605-1-50) and the number of cells secreting IL-17A/F was analyzed by ELISpot.
PBMC were incubated for 48 h in the absence (250,000 cells/well) or presence (50,000 cells/well) of anti-CD3 monoclonal antibody (available from Mabtech, code 3605-1-50) and the number of cells secreting IL-17A/F was analyzed by ELISpot.

Datasheet/ProtocolSDS

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IL-17A/F

Analyte description

Interleukin 17A/F (IL-17A/F) is a heterodimer composed of an IL-17A subunit and an IL-17F subunit. It is a proinflammatory cytokine produced by activated Th17 (T helper 17) cells and certain immune cells of the innate immune system. 

Alternative namesInterleukin 17A/F, IL-17A/F, IL17A/F, CANDF6, IL-17F, IL17A, ML-1, ML1, CTLA-8, CTLA8, IL-17, IL-17A, IL17, ILA17
Cell typeTh17
Gene ID112744, 3605