ELISpot Plus: Mouse IFN-γ (ALP)

ELISpot Plus: Mouse IFN-γ (ALP)

3321-4APT-2

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The easiest way to achieve reproducibility in ELISpot.

Components

Detection mAbR4-6A2, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus
PlatePre-coated ELISpot plate, clear MSIP (mAb AN18)

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting mouse IFN-γ using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Mouse IFN-γ (ALP)
ApplicationELISpot
AnalyteIFN-γ
ReactivityMouse
Specificity

This kit is based on a matched pair of mAbs specific for mouse IFN-γ.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
Mouse spleen cells (150,000 cells/well) were incubated overnight in the absence or presence of Concanavalin A (ConA) and the number of cells secreting IFN-γ was analyzed by ELISpot.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, If2f, Ifg
Cell typeT cell, Tc, Th1, NK cell
Gene ID15978