ELISpot Path: SARS-CoV-2 (RBD) Human IgG (HRP)

ELISpot Path: SARS‑CoV-2 (RBD) Human IgG (HRP)


Publications: 20
A straightforward setup for functional analysis of a specific scientific question.


PlatePre-coated ELISpot plate, white MSIP (mAbs MT91/145)
Detection mAbMT78/145, biotin
Enzyme conjugatesStreptavidin-HRP
Anti-WASP mAb, HRP
SubstrateELISpot substrate: TMB for HRP
Buffer/SolutionReconstitution buffer

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Product specifications

Intended use

This ELISpot Path kit is intended for the enumeration of B cells secreting human IgG in response to SARS-CoV-2 (RBD) using the ELISpot assay. B cells secreting IgG irrespective of antigen specificity (total IgG) can also be enumerated. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

For memory B cells, we recommend pre-stimulation with R848 and recombinant human IL-2 (both included).


Product details

ProductELISpot Path: SARS-CoV-2 (RBD) Human IgG (HRP)

This kit is based on a matched pair of mAbs specific for the Fc part of human IgG.

Shipping and Storage


Shipped at ambient temperature.


Store antibodies, enzyme conjugate, and substrate at 4-8 °C. Recombinant protein should be stored at -20 °C or below. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
Human PBMC from a COVID-19 convalescent individual and a healthy control were incubated in the presence and absence of R848+IL-2 (stimulates memory B cells) in tubes for three days. Cells were then extensively washed and added to an ELISpot plate (200,000 cells/well) coated with anti-human IgG mAbs. RBD-specific spots were detected with RBD-WASP, i.e., recombinant Receptor Binding Domain protein with a WASP-tag, followed by anti-WASP-HRP and the number of cells secreting RBD-specific IgG was analyzed by ELISpot.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.
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Analyte description

Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.

Alternative namesImmunoglobulin G, IgG, IgG3
Cell typeB cell
Gene ID3500, 3501, 3502