ELISpot Path: SARS‑CoV‑2 (RBD) Human IgG (HRP)

Product discontinued

This product is no longer available for purchase.
You may be interested in the following alternative:

ELISpot Path: SARS-CoV-2 (RBD) Human IgG (ALP)

Human PBMC from a COVID-19 convalescent individual and a healthy control were incubated in the presence and absence of R848+IL-2 (stimulates memory B cells) in tubes for three days. Cells were then extensively washed and added to an ELISpot plate (200,000 cells/well) coated with anti-human IgG mAbs. RBD-specific spots were detected with RBD-WASP, followed by anti-WASP-HRP and the number of cells secreting RBD-specific IgG was analyzed by ELISpot

ELISpot Path: SARS‑CoV‑2 (RBD) Human IgG (HRP)

Product discontinued

This product is no longer available for purchase.
You may be interested in the following alternative:

ELISpot Path: SARS-CoV-2 (RBD) Human IgG (ALP)

This discontinued product was available in the following format:

1 plate (Product code: 3850-4HPW-R1-1)

Components

Plate	Pre-coated ELISpot plate, white MSIP (mAbs MT91/145)
Detection mAb	MT78/145, biotin
Antigen	RBD-WASP
Enzyme conjugates	Streptavidin-HRP enzyme conjugate
	Anti-WASP mAb, HRP
Substrate	TMB substrate for ELISpot
Buffer/Solution	Reconstitution buffer

Product specifications

Intended use

This ELISpot Path kit is intended for the enumeration of B cells secreting human IgG in response to SARS-CoV-2 (RBD) using the ELISpot assay. B cells secreting IgG irrespective of antigen specificity (total IgG) can also be enumerated. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

For memory B cells, we recommend pre-stimulation with R848 and recombinant human IL-2 (both included).

Recommendation

Product details

Product	ELISpot Path: SARS-CoV-2 (RBD) Human IgG (HRP)
Application	ELISpot
Analyte	IgG
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for the Fc part of human IgG.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, enzyme conjugate, and substrate at 4-8 °C. Recombinant protein should be stored at -20 °C or below. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Performance

Please be advised that this product has been discontinued.

Human PBMC from a COVID-19 convalescent individual and a healthy control were incubated in the presence and absence of R848+IL-2 (stimulates memory B cells) in tubes for three days. Cells were then extensively washed and added to an ELISpot plate (200,000 cells/well) coated with anti-human IgG mAbs. RBD-specific spots were detected with RBD-WASP, i.e., recombinant Receptor Binding Domain protein with a WASP-tag, followed by anti-WASP-HRP and the number of cells secreting RBD-specific IgG was analyzed by ELISpot.

Product highlights