I have compared Mabtech ELISpot assay to ELISpot assays we run at our company on regular basis "head-to-head" using identical samples. Mabtech's kits proved to be superior to our protocol, had significantly lower background and provided better and higher detection of spots.
PVDF membrane plates for optimal performance
These MSIP plates (MSIPS4W10 from Millipore) are sterile MultiscreenHTSIP plates suitable for ELISpot assays. Plates are white and equipped with an underdrain. For optimal binding of capture antibody, it is recommended to pre-wet, i.e. activate, the PVDF membrane with ethanol.
For more information on ethanol treatment: visit our Plate coating guide.
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