ELISpot Plus: Rat IL-22 (HRP)

ELISpot Plus: Rat IL-22 (HRP)


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The easiest way to achieve reproducibility in ELISpot.


PlatePre-coated ELISpot plate, white MSIP (mAb MT230)
Detection mAbMT231, biotin
Enzyme conjugateStreptavidin-HRP
SubstrateELISpot substrate: TMB for HRP

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Delivery 4-9 business days

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Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting rat IL-22 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples


Product details

ProductELISpot Plus: Rat IL-22 (HRP)
SpecificityThis kit is based on a matched pair of mAbs enabling specific detection of rat IL-22.
Cross-reactivityThe mAbs react with mouse IL-22 and cross-react with IL-22 from rat. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 18 months from date of receipt.
Rat spleen cells were incubated overnight in the absence or presence of PMA/ionomycin and the number of cells secreting IL-22 was analyzed by ELISpot.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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Analyte description

The cytokine interleukin 22 (IL-22) is mainly produced by activated CD4 T helper 17 (Th17) and T helper 22 (Th22) cells. The IL-22 receptor is expressed on non-immune cells, particularly epithelial cells and keratinocytes, and IL-22 promotes innate immune responses versus bacterial infections in these target cells. In addition, Th22 cells have been shown to play a role in the pathophysiology of several human skin diseases.

Alternative namesInterleukin-22, IL-22, IL22, IL-22a, ILTIFa, If2b1, Iltif
Cell typeT cell, Th1, Th17, Tfh
Gene ID50929