ELISA Flex: Rabbit IgG (HRP)

ELISA Flex: Rabbit IgG (HRP)

3152-1H-6

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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMT5131
Detection mAbMT536, biotin
Enzyme conjugateStreptavidin-HRP
StandardRabbit IgG ELISA standard
Buffer/SolutionStandard reconstitution buffer

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Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of rabbit IgG in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISA Flex: Rabbit IgG (HRP)
ApplicationELISA
AnalyteIgG
ReactivityRabbit
SpecificityThis kit is based on a matched pair of mAbs specific for rabbit IgG.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf lifeAt least 18 months from date of receipt.
Standard range20-2500 pg/ml
Representative standard curve for rabbit IFN-γ ELISA Flex kit using pNPP substrate.

Tutorial, Published November 28, 2022

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IgG

Analyte description

Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.

Alternative namesImmunoglobulin G, IgG
Cell typeB cell