Anti-human apoE mAb (E887), biotin

Anti-human apoE mAb (E887), biotin

3712-6-250

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Content

Monoclonal antibody E887, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Apo ELISA buffer
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  • Code: 3652-M2
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Product specifications

Intended use

This monoclonal antibody enables specific detection of human apoE in immunoassays such as ELISA, ELISpot and Western blot. The antibody is also suitable for immunoprecipitation.

Serum/Plasma samples

Recommendation

E887 is recommended as detection mAb in ELISA and ELISpot in combination with capture mAb E276 (product code 3712-3) for human apoE. For monkey apoE, E887 should be used in combination with capture mAb E981 (product code 3712M-3).

Product details

ProductAnti-human apoE mAb (E887), biotin
ApplicationELISpot, ELISA, Western blot
AnalyteapoE
AntibodyE887
ConjugateBiotin
ClonalityMonoclonal
ImmunogenNative human apoE
HostMouse
IsotypeIgG1
ReactivityHuman, Non-human primates
Specificity

Native and recombinant human apolipoprotein E (isoforms apoE2, apoE3, and apoE4). The presence of low amounts of detergent is necessary for binding. Tween 20, Triton X100 or NP40 can be used at a concentration of 0.01-0.5%. Do not vortex with detergent added.

Cross-reactivity

Cross-reacts with native apoE from rhesus and cynomolgus macaques. For more information please check the Cross-reactivity guide section below.

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody E887, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
Detection of human apoE with mAb E887 in Western blot. Human plasma (0.1 µl and 0.01 µl) in SDS sample buffer were denatured at 70˚C for 10 minutes and run on SDS-page / Western blot. ApoE was detected using 1 µg/ml E887 (biotinylated) followed by SA-ALP and BCIP/NBT-plus substrate.

apoE

Analyte description

Apolipoprotein E (apoE, also known as apolipoproteinE and apo E) is a 34 kD glycoprotein involved in lipid transport, lipoprotein metabolism and cellular signaling. It is present in plasma lipoprotein particles such as chylomicrons, VLDL and HDL. ApoE is also present in large amounts at sites of neurological damage and appears to be involved in recycling of apoptotic remnants and of amyloidal aggregates. ApoE is mainly produced in the liver and by macrophages, but also by other cell types such as smooth muscle cells or neuronal cells. In human apoE exists in three isoforms, apoE2, apoE3 and apoE4. Of these, apoE4 is associated with Alzheimer’s disease. Normal concentration of apoE in human plasma is approximately 30-40 µg/ml. The concentration of ApoE in inbred mouse plasma can vary between 15-150 µg/ml.

Alternative namesApolipoprotein E, AD2, APO-E, ApoE4, LDLCQ5, LPG
Cell typeT cell, Treg, Tfh
Gene ID348
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Tutorial, Published November 14, 2022

Apolipoprotein dilution
Recommendations for Mabtech's apolipoprotein ELISAs