ELISpot Flex: Mouse IgG2c (ALP)

3824-2A

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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAb	MT24/JC5-1
Detection mAb	MTG2c, biotin
Enzyme conjugate	Streptavidin-ALP
Stimuli	R848
	Recombinant mouse IL-2

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Delivery 4-9 business days

Shipping $0

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Anti-mouse IgG2c mAb (MTG2c), unconjugated

Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting mouse IgG2c using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

With this kit, detection of antigen-specific IgG2c secreting B cells can be performed using coated antigen. If detection with biotinylated antigen is preferred, the plate should instead be coated with the subclass specific antibody.

The IgG2c allele is expressed in certain inbred mouse strains e.g., C57BL/6, C57BL/10, SJL and NOD.

For memory B cells, we recommend pre-stimulation with R848 and recombinant mouse IL-2 (both included).

Recommendation

Product details

Product	ELISpot Flex: Mouse IgG2c (ALP)
Application	ELISpot
Analyte	IgG2c
Reactivity	Mouse
Specificity	This kit is based on a matched pair of mAbs enabling specific detection of mouse IgG2c.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. R848 and IL-2 should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Performance

Splenocytes from C57/BL6 mice were pre-incubated for 72 h in the absence or presence of R848 and recombinant IL-2. The cells were then washed, added into ELISpot wells and analyzed for the secretion of IgG2c (50,000 cells/well, overnight incubation).

Documents

Datasheet/Protocol
SDS

Mouse IgG2c mAb guide

Selecting the appropriate mAbs is key to detecting both total and antigen-specific mouse IgG2c. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect mouse IgG2c antibody-secreting cells.

Application guide: ELISpot

Antigen-specific IgG2c

Coating:
Antigen
Detection mAb:
MTG2c, biotin

Coating mAbs:
MT24/JC5-1, unconjugated
Detection:
Antigen

Total IgG2c

Coating mAbs:
MT24/JC5-1, unconjugated
Detection mAb:
MTG2c, biotin

Tutorials

Tutorial, Published December 28, 2023

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Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 21, 2022

B cell profiling with ELISpot and FluoroSpot

ELISpot and FluoroSpot are considered the gold standard method for finding rare, antigen-specific B cells.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

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Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published October 14, 2022

Isolation, freezing, and thawing of splenocytes

This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.

Publications (0)

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Analyte information

IgG2c

Analyte description	IgG (Immunoglobulin G) is the most abundant Ig isotype in serum, making up approximately 80% of all serum Immunoglobulins. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG2c allele is expressed (instead of IgG2a) in certain inbred mouse strains, e.g., C57BL/6, C57BL/10, SJL, and NOD.
Alternative names	Immunoglobulin G2c, IgG2c
Cell type	B cell