FluoroSpot Plus: Mouse IFN-γ/IL-2

FSP-4142-2

Ensure reproducibility when diving into a multiplex analysis.

Components

Detection mAbs	Anti-IFN-y mAb (R4-6A2), BAM
	Anti-IL-2 mAb (5H4), biotin
Fluorophore conjugates	Anti-BAM mAb, 490
	SA-550

Co-stimulus	Anti-CD28 mAb
Buffer/Solution	FluoroSpot enhancer
Plate	Pre-coated FluoroSpot plate (mAbs AN18 and 1A12)

In stock

Delivery 4-9 business days

Shipping $0.00

Product specifications

Intended use

This FluoroSpot Plus kit is intended for the enumeration of cells secreting mouse IFN-γ and IL-2 using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	FluoroSpot Plus: Mouse IFN-γ/IL-2
Application	FluoroSpot
Analyte	IFN-γ, IL-2
Reactivity	Mouse
Specificity	This kit is based on matched pairs of mAbs specific for mouse IFN-γ and IL-2.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Performance

Mouse spleen cells (125,000 cells/well) were incubated overnight in the absence or presence of Concanavalin A (ConA) and the number of cells secreting IFN-γ and/or IL-2 was analyzed by FluoroSpot. Single cytokine secreting cells as well as double-secreting cells were determined. Spot center was used to identify spots from double-secreting cells, visualized in an image overlay of LED490, FITC (IFN-γ) and LED550 and Cy3 (IL-2). Spot analysis was made with Mabtech IRIS.

Documents

Datasheet/Protocol

SDS

Tutorial

Tutorial, Published October 10, 2022

Step-by-step guide to FluoroSpot

An in-depth guide to FluoroSpot, including best practice and tricks we've learnt over the years.

Tutorial, Published October 14, 2022

Isolation, freezing, and thawing of splenocytes

This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.