FluoroSpot Plus: Mouse IFN-γ/IL-2

FluoroSpot Plus: Mouse IFN‑γ/IL‑2

FSP-4142-2

Publications: 15
Documents
Questions?
Ensure reproducibility when diving into a multiplex analysis.

Components

PlatePre-coated FluoroSpot plate (mAbs AN18 and 1A12)
Detection mAbsAnti-IFN-γ mAb (R4-6A2), BAM
Anti-IL-2 mAb (5H4), biotin
Fluorophore conjugatesAnti-BAM mAb, 490
SA-550
Buffer/SolutionFluoroSpot enhancer
Co-stimulusAnti-CD28 mAb

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This FluoroSpot Plus kit is intended for the enumeration of cells secreting mouse IFN-γ and IL-2 using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductFluoroSpot Plus: Mouse IFN-γ/IL-2
ApplicationFluoroSpot
AnalyteIFN-γ, IL-2
ReactivityMouse
SpecificityThis kit is based on matched pairs of mAbs specific for mouse IFN-γ and IL-2.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 12 months from date of receipt.
Mouse spleen cells (125,000 cells/well) were incubated overnight in the absence or presence of Concanavalin A (ConA) and the number of cells secreting IFN-γ and/or IL-2 was analyzed by FluoroSpot. Single cytokine secreting cells as well as double-secreting cells were determined. Spot center was used to identify spots from double-secreting cells, visualized in an image overlay of LED490, FITC (IFN-γ) and LED550 and Cy3 (IL-2). Spot analysis was made with Mabtech IRIS.
Datasheet/Protocol
SDS

Highlighted research, Published October 11, 2024

Immunoassays for vaccines
Explore the critical role of immunoassays like ELISpot and FluoroSpot in vaccine development, from evaluating immune responses to tracking vaccine efficacy and potency. These assays provide sensitive, single-cell insights for advancing research and development of viral, bacterial, and cancer vaccines.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published October 14, 2022

Isolation, freezing, and thawing of splenocytes
This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, If2f, Ifg
Cell typeT cell, Tc, Th1, NK cell
Gene ID15978

IL-2

Analyte description

Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases. 

Alternative namesInterleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, Il-2
Cell typeT cell, Th1, Th2, Th17, Tfh
Gene ID16183
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FluoroSpot Plus: Mouse IFN-γ/IL-2/TNF-α