FluoroSpot Plus: Mouse IFN-γ/IL-2

FluoroSpot Plus: Mouse IFN-γ/IL-2


Ensure reproducibility when diving into a multiplex analysis.


Detection mAbsAnti-IFN-y mAb (R4-6A2), BAM
Anti-IL-2 mAb (5H4), biotin
Fluorophore conjugatesAnti-BAM mAb, 490
Co-stimulusAnti-CD28 mAb
Buffer/SolutionFluoroSpot enhancer
PlatePre-coated FluoroSpot plate (mAbs AN18 and 1A12)

In stock

Delivery 4-9 business days

Shipping $0.00

Product specifications

Intended use

This FluoroSpot Plus kit is intended for the enumeration of cells secreting mouse IFN-γ and IL-2 using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples


Product details

ProductFluoroSpot Plus: Mouse IFN-γ/IL-2
AnalyteIFN-γ, IL-2
SpecificityThis kit is based on matched pairs of mAbs specific for mouse IFN-γ and IL-2.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 12 months from date of receipt.
Mouse spleen cells (125,000 cells/well) were incubated overnight in the absence or presence of Concanavalin A (ConA) and the number of cells secreting IFN-γ and/or IL-2 was analyzed by FluoroSpot. Single cytokine secreting cells as well as double-secreting cells were determined. Spot center was used to identify spots from double-secreting cells, visualized in an image overlay of LED490, FITC (IFN-γ) and LED550 and Cy3 (IL-2). Spot analysis was made with Mabtech IRIS.