ELISA Flex: Equine IL-10 (HRP)

ELISA Flex: Equine IL-10 (HRP)

3126-1H-6

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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMT3515
Detection mAbMT32AD, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant human IL-10 ELISA standard
Buffer/SolutionStandard reconstitution buffer

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Delivery 4-9 business days

Shipping $0

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ELISA substrate: TMB for HRP


Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of equine IL-10 in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in serum/plasma from other species. The ELISA diluent has been validated using from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

ProductELISA Flex: Equine IL-10 (HRP)
ApplicationELISA
AnalyteIL-10
ReactivityHorse
Specificity

This kit is based on a matched pair of mAbs enabling specific detection of equine (horse) IL-10.

Cross-reactivity

The mAbs react with monkey IL-10 and cross-react with IL-10 from human and horse. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range2-250 pg/ml
Representative standard curve for equine IL-10 ELISA Flex kit using TMB substrate.
Datasheet/Protocol
SDS

Behind the scenes, Published March 27, 2023

We received an award
We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category. 

Featured product, Published April 28, 2022

Expanding our horse portfolio with IL-10 kits
New IL-10 antibodies for horse research out now.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA
Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Tutorial, Published July 31, 2023

ELISA publications by sample type
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IL-10

Analyte description

Interleukin 10 (IL-10) is a pleiotropic cytokine produced by activated T cells and monocytes. It can modulate the functions of immune responses in both a stimulatory and an inhibitory way. IL-10 is thereby involved in the regulation of immune reactions and inflammatory responses.

Alternative namesInterleukin-10, IL-10, IL10
Cell typeT cell, Treg, Monocyte/MΦ, mDC