ELISA Flex: Porcine TNF-α (HRP)

ELISA Flex: Porcine TNF-α (HRP)

3135-1H-6

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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMT1P14
Detection mAbMT859, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant porcine TNF-α ELISA standard
Buffer/SolutionStandard reconstitution buffer

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ELISA substrate: TMB for HRP


Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of porcine TNF-α in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISA Flex: Porcine TNF-α (HRP)
ApplicationELISA
AnalyteTNF-α
ReactivityPig
SpecificityThis kit is based on a matched pair of mAbs specific for porcine (pig) TNF-α.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf lifeAt least 18 months from date of receipt.
Standard range10-1000 pg/ml
Representative standard curve for porcine TNF-α ELISA Flex kit using pNPP substrate.
Datasheet/Protocol
SDS

Behind the scenes, Published March 27, 2023

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Tutorial, Published November 28, 2022

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Tutorial, Published July 31, 2023

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TNF-α

Analyte description

Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Alternative namesTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF
Cell typeT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ