ELISA Flex: Mouse IgM (ALP)

ELISA Flex: Mouse IgM (ALP)


Publications: 8
An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.


Capture mAbMT6A3
Detection mAbMT9A2, biotin
Enzyme conjugateStreptavidin-ALP
StandardNative mouse IgM ELISA standard
Buffer/SolutionStandard reconstitution buffer

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Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of mouse IgM in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples


Product details

ProductELISA Flex: Mouse IgM (ALP)
SpecificityThis kit is based on a matched pair of mAbs enabling specific detection of mouse IgM.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf lifeAt least 18 months from date of receipt.
Standard rangeRecommended standard dilution 0.1-20 ng/ml
Representative standard curve for mouse IgM ELISA Flex kit using pNPP substrate.

Tutorial, Published July 31, 2023

ELISA publications by sample type
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Analyte description

Immunoglobulin M (IgM) is the first antibody isotype (also called class) secreted by the adaptive immune system in response to a foreign antigen. Plasma B cells secrete IgM mainly as pentamers, i.e., five IgM molecules joined together by the J chain. Because IgM is formed early in the immune response and is later replaced by IgG, specific antibodies of the IgM class are characteristic of a recent, ongoing, or chronic infection.  Therefore, IgM represents the isotype with the lowest affinity for antigens. Due to its structure, with 10 antigen-binding sites per IgM pentamer, it can nevertheless bind surface-bound antigens with very high avidity. Immunoglobulin M constitutes approximately 10% of the total immunoglobulin concentration in serum. Resting mature yet naive, B lymphocytes express monomeric IgM as a transmembrane antigen receptor that functions as part of the B cell receptor (BCR).

Alternative namesImmunoglobulin M, IgM, AI326478, Igh-6, Igh-M, Igh6, Igm, muH
Cell typeB cell
Gene ID16019