ELISA Flex: Human IFN-α (HRP)

ELISA Flex: Human IFN-α (HRP)


Publications: 123
An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.


Capture mAbMT1/3/5
Detection mAbMT2/4/6, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant human IFN-α ELISA standard
Buffer/SolutionStandard reconstitution buffer

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ELISA diluent
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  • Code: 3652-D2
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Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human IFN-α in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in serum/plasma from other species. The ELISA diluent has been validated using from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.


Product details

ProductELISA Flex: Human IFN-α (HRP)
AnalyteIFN-α pan
ReactivityHuman, Non-human primates

This kit is based on a matched pair of mAbs enabling specific detection of human IFN-α. Subtypes 1/13, 2, 4, 5, 6, 7, 8, 10, 14, 16, and 17 are detected.


The mAbs cross-react with IFN-α from non-human primates (NHP). For more information please check the Cross-reactivity guide section below.

Shipping and Storage


Shipped at ambient temperature.


Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range4-400 pg/ml
Representative standard curve for human IFN-α ELISA Flex kit using TMB substrate.

IFN-α pan

Analyte description

Interferon-α and interferon-β are type I interferons. These antiviral cytokines are mainly produced by virus-infected cells after sensing the presence of danger signals such as viral DNA or RNA. IFN-α and IFN-β act through the same receptor and initiate an anti-viral response, affect immune regulation, T cell responses, and also anti-tumor effects have been shown. A key producer of type I interferons is the plasmacytoid dendritic cell. Additionally, IFN-α is secreted by lymphocytes, macrophages, fibroblasts, and dendritic cells. 

In humans, interferon-α has 13 subtypes: IFN-α 1, 2, 4, 5, 6, 7, 8, 10, 13, 14, 16, 17, and 21.

In mice, there are 14 IFN-α subtypes: 1, 2, 4, 5, 6T, 7/10, 8/6, 9, 11, 12, 13, 14, 15 (A), and B. There are also different allelic forms of mouse IFN-α subtypes 1, 7/10, 8/6, and 11 in mouse strains 129/Sv and C57BL/6.

Alternative namesInterferon-α, Interferon-alpha, IFN-α, IFN-alpha, IFN-a, IFNA
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