|Detection mAb||MT2/4/6, biotin|
|Standard||Recombinant human IFN-α ELISA standard|
|Buffer/Solution||Standard reconstitution buffer|
Delivery 4-9 business days
This ELISA Flex kit is intended for the quantification of human IFN-α in solution. For research use only. Not for use in diagnostic procedures.
Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in serum/plasma from other species. The ELISA diluent has been validated using from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.
|Product||ELISA Flex: Human IFN-α (HRP)|
|Reactivity||Human, Non-human primates|
This kit is based on a matched pair of mAbs enabling specific detection of human IFN-α. Subtypes 1/13, 2, 4, 5, 6, 7, 8, 10, 14, 16, and 17 are detected.
The mAbs cross-react with IFN-α from non-human primates (NHP). For more information please check the Cross-reactivity guide section below.
Shipping and Storage
Shipped at ambient temperature.
Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
|Shelf life||At least 18 months from date of receipt.|
|Standard range||4-400 pg/ml|
Interferon-α and interferon-β are type I interferons. These antiviral cytokines are mainly produced by virus-infected cells after sensing the presence of danger signals such as viral DNA or RNA. IFN-α and IFN-β act through the same receptor and initiate an anti-viral response, affect immune regulation, T cell responses, and also anti-tumor effects have been shown. A key producer of type I interferons is the plasmacytoid dendritic cell. Additionally, IFN-α is secreted by lymphocytes, macrophages, fibroblasts, and dendritic cells.
|Alternative names||Interferon-α, Interferon-alpha, IFN-α, IFN-alpha, IFN-a, IFNA|