Anti-porcine TNF-α mAb (MT32E9), unconjugated

Anti‑porcine TNF-α mAb (MT32E9), unconjugated

3135-3-250

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Content

Monoclonal antibody MT32E9, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Product specifications

Intended use

This monoclonal antibody enables specific detection of porcine TNF-α in ELISpot and FluoroSpot.

Serum/Plasma samples

Recommendation

MT32E9 is recommended as capture mAb in ELISpot and FluoroSpot in combination with detection mAb MT859 (product code 3135-6).

Product details

ProductAnti-porcine TNF-α mAb (MT32E9), unconjugated
ApplicationELISpot, FluoroSpot
AnalyteTNF-α
AntibodyMT32E9
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenRecombinant porcine TNF-α
HostMouse
IsotypeIgG1
ReactivityPig
SpecificityNative and recombinant porcine (pig) TNF-α
PurificationPurified from in vitro cultures by protein G affinity chromatography.
Concentration0.5 mg/ml
Supplied inPBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibody MT32E9, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf lifeAt least 18 months from date of receipt.

Find out which analyte combinations we have evaluated in T cell FluoroSpot and which combinations are affected by capture effects or not. 

What is a capture effect?

When capture antibodies with different specificities are coated together, capture of one cytokine may affect the secretion of other cytokines. This is usually more pronounced when studying T cell responses with polyclonal stimuli compared to antigen-specific responses. Capture effects are seldom a problem and can often be counteracted by addition of an anti-CD28 antibody.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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TNF-α

Analyte description

Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Alternative namesTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF
Cell typeT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
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