Anti-mouse TNF-α mAb (MT11B10), biotin

3511-6-250

Publications: 63

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Anti-mouse TNF-α mAbs (MT1C8/23C9), unconjugated

Intended use

This monoclonal antibody enables specific detection of mouse TNF-α in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples

Recommendation

MT11B10 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAbs MT1C8/23C9 (product code 3511-3).

Product details

Product	Anti-mouse TNF-α mAb (MT11B10), biotin
Application	ELISpot, FluoroSpot, ELISA
Analyte	TNF-α
Antibody	MT11B10
Conjugate	Biotin
Clonality	Monoclonal
Immunogen	Recombinant mouse TNF-α
Host	Rat
Isotype	IgG2a
Reactivity	Mouse
Specificity	Native and recombinant mouse TNF-α
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MT11B10, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

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TNF-α

Analyte description	Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.
Alternative names	Tumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF, TNFSF2, TNFalphaa, Tnfsf1a, Tnlg1f, Tnf
Cell type	T cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID	21926

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ELISA Pro: Mouse TNF-α