Delivery 4-9 business days
Intended useThis monoclonal antibody enables specific detection of mouse IL-4 in immunoassays such as ELISpot, FluoroSpot, and ELISA.
Recommendation11B11 is recommended as capture mAb in ELISpot, FluoroSpot, and ELISA in combination with detection mAb BVD6-24G2 (product code 3311-6).
|Product||Anti-mouse IL-4 mAb (11B11), unconjugated|
|Application||ELISpot, FluoroSpot, ELISA|
|Immunogen||Recombinant mouse IL-4|
|Specificity||Native and recombinant mouse IL-4|
|Purification||Purified from in vitro cultures by protein G affinity chromatography.|
|Supplied in||PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).|
|Contents||Monoclonal antibody 11B11, unconjugated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.|
Shipping and Storage
|Shipping||Shipped at ambient temperature.|
|Storage||Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.|
|Shelf life||At least 18 months from date of receipt.|
Interleukin 4 (IL-4) is produced primarily by Th2 cells, mast cells, eosinophils, and basophils. IL-4 was first identified to costimulate B cell growth; the cytokine is essential for B cell secretion of IgE and augments the production of IgG1 in vivo. In addition to regulating B cell growth and immunoglobulin secretion, IL-4 also affects T cells. In vitro, IL-4 promotes T cell growth and can induce cytolytic T cell activity. IL-4 also induces the differentiation of naive CD4 T cells into Th2 cells, which are characterized by their capacity to secrete the cytokines IL-4, IL-5, and IL-10 upon activation, while simultaneously inhibiting the generation of Th1 cells.
|Alternative names||Interleukin 4, IL-4, IL4, BCGF-1, BSF-1, Il-4|
|Cell type||T cell, Th2, Treg|