Anti-human/monkey apoE mAb (E981), unconjugated

Anti-human/monkey apoE mAb (E981), unconjugated


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Validated for our assays.


Monoclonal antibody E981, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-human apoE mAb (E887), biotin
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  • Application:
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  • Code: 3712-6-250
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Product specifications

Intended use

This monoclonal antibody enables specific detection of monkey apoE in immunoassays such as ELISA and Western blot.

Serum/Plasma samples


E981 is recommended as capture mAb in ELISA in combination with detection mAb E887 (product code 3712-6). The antibody is also suitable for immunoprecipitation and Western blot.

Product details

ProductAnti-human/monkey apoE mAb (E981), unconjugated
ApplicationELISA, Western blot
ImmunogenNative human apoE
ReactivityNon-human primates

Native apolipoprotein E (isoforms apoE2, apoE3, and apoE4) from rhesus and cynomolgus macaques. Native and recombinant apoE from human. The presence of low amounts of detergent is necessary for binding. Tween 20, Triton X100 or NP40 can be used at a concentration of 0.01-0.5%. Do not vortex with detergent added.


Purified from in vitro cultures by protein G affinity chromatography.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).


Monoclonal antibody E981, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage


Shipped at ambient temperature.


Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
Detection of human apoE with mAb E981 in Western blot. Human plasma (0.1 µl and 0.01 µl) in SDS sample buffer were denatured at 70˚C for 10 minutes and run on SDS-page / Western blot. ApoE was detected using 1 µg/ml E981 (biotinylated) followed by SA-ALP and BCIP/NBT-plus substrate.


Analyte description

Apolipoprotein E (apoE, also known as apolipoproteinE and apo E) is a 34 kD glycoprotein involved in lipid transport, lipoprotein metabolism and cellular signaling. It is present in plasma lipoprotein particles such as chylomicrons, VLDL and HDL. ApoE is also present in large amounts at sites of neurological damage and appears to be involved in recycling of apoptotic remnants and of amyloidal aggregates. ApoE is mainly produced in the liver and by macrophages, but also by other cell types such as smooth muscle cells or neuronal cells. In human apoE exists in three isoforms, apoE2, apoE3 and apoE4. Of these, apoE4 is associated with Alzheimer’s disease. Normal concentration of apoE in human plasma is approximately 30-40 µg/ml. The concentration of ApoE in inbred mouse plasma can vary between 15-150 µg/ml.

Alternative namesApolipoprotein E, AD2, APO-E, ApoE4, LDLCQ5, LPG
Cell typeT cell, Treg, Tfh
Gene ID348
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