Anti-human IL-1β mAb (7P10), biotin

Anti-human IL-1β mAb (7P10), biotin

3416-6-250

Publications: 40
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Content

Monoclonal antibody 7P10, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Delivery 4-9 business days

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Product specifications

Intended use

This monoclonal antibody enables specific detection of human IL-1β in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples

Recommendation

7P10 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb MT175 (product code 3416-3).

Product details

ProductAnti-human IL-1β mAb (7P10), biotin
ApplicationELISpot, FluoroSpot, ELISA
AnalyteIL-1β
Antibody7P10
ConjugateBiotin
ClonalityMonoclonal
ImmunogenRecombinant human IL-1β
HostMouse
IsotypeIgG2b
ReactivityHuman
Specificity

Native and recombinant human IL-1β. The antibody binds to the pro-form and the active form of IL-1β.

Cross-reactivity

No cross-reactivity with human IL-1α

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody 7P10, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C.

Shelf lifeAt least 18 months from date of receipt.

Find out which analyte combinations we have evaluated in T cell FluoroSpot and which combinations are affected by capture effects or not. 

What is a capture effect?

When capture antibodies with different specificities are coated together, capture of one cytokine may affect the secretion of other cytokines. This is usually more pronounced when studying T cell responses with polyclonal stimuli compared to antigen-specific responses. Capture effects are seldom a problem and can often be counteracted by addition of an anti-CD28 antibody.

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IL-1β

Analyte description

Interleukin 1ß (IL-1ß) is a proinflammatory cytokine and inducer of acute phase responses. IL-1ß is produced primarily by monocytes, macrophages, and dendritic cells after induction by microbes.

Alternative namesInterleukin-1ß, IL-1ß, IL-1F2, Interleukin-1beta, IL-1 beta, IL1b, Interleukin-1 beta, IL-1, IL1-BETA, IL1F2, IL1beta
Cell typeMonocyte/MΦ, mDC
Gene ID3553
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