Anti-human apoB mAbs (LDL 20/17), unconjugated

3715-3-250

Publications: 74

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Content

Monoclonal antibodies LDL 20/17, unconjugated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

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Anti-human apoB mAb (LDL 11), biotin

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Code: 3715-6-250
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Product specifications

Intended use

These monoclonal antibodies enable specific detection of human apoB in immunoassays such as ELISA and Western blot.

Serum/Plasma samples

Recommendation

LDL 20/17 are recommended as capture mAbs in ELISA in combination with detection mAb LDL 11 (product code 3715-6). The antibodies are also suitable for immunoprecipitation and Western blot.

Product details

Product	Anti-human apoB mAbs (LDL 20/17), unconjugated
Application	ELISA, Western blot
Analyte	apoB
Antibody	LDL 20/17
Conjugate	Unconjugated
Clonality	Monoclonal
Immunogen	Native human LDL
Host	Mouse
Isotype	IgG2a
Reactivity	Human, Non-human primates
Specificity	Native human apoB in the form of LDL
Cross-reactivity	Cross-react with apoB from rhesus and cynomolgus macaques. For more information please check the Cross-reactivity guide section below.
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Concentration	1 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibodies LDL 20/17, unconjugated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Performance

Detection of human apoB with mAb LDL20/17 in Western blot. Human plasma (0.1 µl and 0.01 µl) in SDS sample buffer were denatured at 70˚C for 10 minutes and run on SDS-page / Western blot. ApoB was detected using 1 µg/ml LDL20/17 (biotinylated) followed by SA-ALP and BCIP/NBT-plus substrate.

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Datasheet/Protocol SDS

Analyte information

apoB

Analyte description	Apolipoprotein B (apoB, also known as apolipoproteinB, apo B, apoB-100, apoB100 and apo B100) is the main protein component of low density lipoprotein particles (LDL) and it is a large water insoluble molecule of 512 kD (apoB-100) produced mainly in the liver and in the intestine (apoB-48). ApoB is regarded as a negative factor in cardiovascular disease. ApoB-100 is normally 98% of the apoB in human plasma and at a concentration of approximately 0.4-1.3 mg/ml.
Alternative names	, FCHL2, FLDB, LDLCQ4, apoB-100, apoB-48
Gene ID	338

Publications (74)

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Tutorials

Tutorial, Published November 14, 2022

Apolipoprotein dilution

Recommendations for Mabtech's apolipoprotein ELISAs

Cross-reactivity guide

Cross-reactivity guide, Published November 21, 2022

Non-human primate cross-reactivity

Mabtech offers a broad range of ELISpot kits in different formats for the detection of cytokines and other immunological effector molecules in samples from non-human primates (NHP).