Anti-bovine IL-17A mAb (MT51B8), biotin

Anti-bovine IL-17A mAb (MT51B8), biotin

3109-6-250

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Content

Monoclonal antibody MT51B8, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This monoclonal antibody enables specific detection of bovine, sheep, and goat IL-17A in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples

Recommendation

MT51B8 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb MT49A7 (product code 3109-3).

Product details

ProductAnti-bovine IL-17A mAb (MT51B8), biotin
ApplicationELISpot, FluoroSpot, ELISA
AnalyteIL-17A
AntibodyMT51B8
ConjugateBiotin
ClonalityMonoclonal
ImmunogenRecombinant bovine IL-17A
HostMouse
IsotypeIgG2b
ReactivityCow, Goat, Sheep
Specificity

Native and recombinant bovine (cow) IL-17A

Cross-reactivity

Cross-reacts with IL-17A from sheep and goat. For more information, please check the Veterinary cross-reactivity guide.

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MT51B8, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

Find out which analyte combinations we have evaluated in T cell FluoroSpot and which combinations are affected by capture effects or not. 

What is a capture effect?

When capture antibodies with different specificities are coated together, capture of one cytokine may affect the secretion of other cytokines. This is usually more pronounced when studying T cell responses with polyclonal stimuli compared to antigen-specific responses. Capture effects are seldom a problem and can often be counteracted by addition of an anti-CD28 antibody.

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IL-17A

Analyte description

Interleukin 17A (IL-17A) is a potent proinflammatory cytokine produced by activated Th17 (T helper 17) cells and certain cells belonging to the innate immune system. In mice, IL-17 has also been shown to be produced by activated CD8 T cells and γδ T cells. Th17 cells play an important role in autoimmune diseases and protection against bacteria and fungi. IL-17A acts on a broad range of cell types to induce the expression of cytokines, chemokines, and metalloproteinases. As a result, secretion of IL-17A promotes inflammatory responses, which leads to the recruitment of neutrophils, enhancement of antibody production, and activation of T cells. Increased expression of IL-17A is seen in autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. It is also associated with asthma, psoriasis, cancer, and transplant rejection.

Alternative namesInterleukin 17A, IL-17A, IL17A, IL-17, IL17
Cell typeTh17
Gene ID282863