ELISAPRO kit for quantification of apoE in solution
The apoE ELISAPRO kit provides all necessary reagents and solutions required to quantify apoE in solution, e.g. in cell culture supernatant, and serum/plasma samples. The three isoforms apoE2, apoE3 and apoE4 are detected. The kit contains ELISA plates pre-coated with monoclonal capture antibody, biotinylated detection antibody, Streptavidin-HRP, TMB substrate, ELISA standard and Apo ELISA buffer concentrate. The Apo ELISA buffer enables analysis of serum/plasma samples without interference from heterophilic antibodies. The kit contains pre-coated plates and matched amount of reagents and solutions.
Dilution guidelines for serum/plasma samples can be found here.
Pre-coated ELISA plates save time and reduce assay variability.
- Handattu S. P., et al. Two apolipoprotein E mimetic peptides with similar cholesterol reducing properties exhibit differential atheroprotective effects in LDL-R null mice Atherosclerosis 227 58, 2013
- Liu M., et al. Apolipoprotein E does not cross the blood-cerebrospinal fluid barrier, as revealed by an improved technique for sampling CSF from mice Am J Physiol Regul Integr Comp Physiol 303 R903, 2012
- Niculescu L. S., et al. HDL inhibit endoplasmic reticulum stress by stimulating apoE and CETP secretion from lipid-loaded macrophages Biochemical and biophysical research communications 434 173, 2013
- Sciacca B., et al. Radiative-surface plasmon resonance for the detection of apolipoprotein E in medical diagnostics applications Nanomedicine 9 550, 2013
Apolipoprotein E (apoE, also known as apolipoproteinE and apo E) is a 34 kD heparin binding glycoprotein involved in lipid transport and lipoprotein metabolism. It is present in plasma lipoprotein particles such as chylomicrons, VLDL and HDL. ApoE is also present in large amounts at sites of neurological damage and appears to be involved in recycling of apoptotic remnants and of amyloidal aggregates. ApoE is mainly produced in the liver and by macrophages, but also by other cell types such as smooth muscle cells or neuronal cells. ApoE exists in three isoforms, apoE2, apoE3 and apoE4. Of these, apoE4 is associated with Alzheimer’s disease. Normal concentration of apoE in human plasma is approximately 30-40 µg/ml.