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3712-1HP-2

Human apoE ELISA PRO kit

  • Human apoE ELISA kit
  • Standard curve for human apoE ELISAPRO kit.
  • Apolipoprotein dilution
$495
-+

ELISAPRO kit for quantification of apoE in solution

The apoE ELISAPRO kit provides all necessary reagents and solutions required to quantify apoE in solution, e.g. in cell culture supernatant, and serum/plasma samples. The three isoforms apoE2, apoE3 and apoE4 are detected. The kit contains ELISA plates pre-coated with monoclonal capture antibody, biotinylated detection antibody, Streptavidin-HRP, TMB substrate, ELISA standard and Apo ELISA buffer concentrate. The Apo ELISA buffer enables analysis of serum/plasma samples without interference from heterophilic antibodies. The kit contains pre-coated plates and matched amount of reagents and solutions.

Dilution guidelines for serum/plasma samples can be found here.

Pre-coated ELISA plates save time and reduce assay variability.

Application:
  • ELISA
Reactivity:
  • Human
Documentation: Product datasheet

Product SDS
Contents:
  • Biotinylated detection mAb (E887)
  • Streptavidin-HRP
  • Recombinant human apoE ELISA standard
  • Substrate (TMB)
  • Buffers for washing, dilution and standard reconstitution
  • 2 Pre-coated ELISA strip plates (mAb E276)
Size: 2 plates
  • Apolipoprotein Dilution

    Guidelines for dilution of serum/plasma samples for quantification of apolipoproteins in ELISA.

    Apolipoprotein dilution  

  • Analyte information

    • ApoE
      Apolipoprotein E (apoE, also known as apolipoproteinE and apo E) is a 34 kD heparin binding glycoprotein involved in lipid transport and lipoprotein metabolism. It is present in plasma lipoprotein particles such as chylomicrons, VLDL and HDL. ApoE is also present in large amounts at sites of neurological damage and appears to be involved in recycling of apoptotic remnants and of amyloidal aggregates. ApoE is mainly produced in the liver and by macrophages, but also by other cell types such as smooth muscle cells or neuronal cells. ApoE exists in three isoforms, apoE2, apoE3 and apoE4. Of these, apoE4 is associated with Alzheimer’s disease. Normal concentration of apoE in human plasma is approximately 30-40 µg/ml.
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