ELISA Pro: Human IL-1β

ELISA Pro: Human IL-1β

3416-1HP-1

Publications: 36
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Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.

Components

Detection mAb7P10, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant human IL-1β ELISA standard
SubstrateELISA substrate: TMB for HRP
PlatePre-coated ELISA strip plate (mAb MT175)
Also providedAdhesive plate covers
Buffers/SolutionsELISA diluent
Standard reconstitution buffer
Streptavidin-HRP diluent
Wash buffer concentrate
Stop solution

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Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA Pro kit is intended for the quantification of human IL-1β in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

The ELISA diluent included in this kit blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in other species. The ELISA diluent has been validated using from healthy human blood donors.

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Product details

ProductELISA Pro: Human IL-1β
ApplicationELISA
AnalyteIL-1β
ReactivityHuman
SpecificityThis kit is based on a matched pair of mAbs specific for human IL-1β (pro-form and active form).
Cross-reactivityThe mAbs do not cross-react with human IL-1α. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.
Shelf lifeAt least 12 months from date of receipt.
Standard range1-316 pg/ml
Sensitivity0.4 pg/ml

The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 3 standard deviations to the mean OD of background wells.
Representative standard curve for human IL-1β ELISA Flex kit using TMB substrate.
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IL-1β

Analyte description

Interleukin 1ß (IL-1ß) is a proinflammatory cytokine and inducer of acute phase responses. IL-1ß is produced primarily by monocytes, macrophages, and dendritic cells after induction by microbes.

Alternative namesInterleukin-1ß, IL-1ß, IL-1F2, Interleukin-1beta, IL-1 beta, IL1b, Interleukin-1 beta, IL-1, IL1-BETA, IL1F2, IL1beta
Cell typeMonocyte/MΦ, mDC
Gene ID3553
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